doi: 10.1007/978-1-4939-0473-0_20.
Biosensors for qualitative and semiquantitative analysis of quorum sensing signal molecules
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- PMID: 24818910
- DOI: 10.1007/978-1-4939-0473-0_20
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Biosensors for qualitative and semiquantitative analysis of quorum sensing signal molecules
Methods Mol Biol.
2014.
Abstract
Biosensors are biological tools that can be used to assay bacterial cultures for quorum sensing signal molecules (QSSMs) both qualitatively and semiquantitatively. QSSMs can be extracted from Pseudomonas aeruginosa cultures using organic solvents and tentatively identified via thin layer chromatography in combination with biosensor overlays. Alternatively, QSSMs can be quantified in spent culture supernatants or solvent extracts using biosensor-based spectrophotometric, luminescence, or fluorescence assays.
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