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. 2014 Aug 1;68(3):403-8.
doi: 10.1016/j.ymeth.2014.04.016. Epub 2014 May 10.

Applying antibiotic selection markers for nematode genetics

Eric Cornes  1 , Cécile A L Quéré  2 , Rosina Giordano-Santini  3 , Denis Dupuy  4
Affiliations

Applying antibiotic selection markers for nematode genetics

Eric Cornes et al. Methods. .

Abstract

Antibiotic selection markers have been recently developed in the multicellular model organism Caenorhabditis elegans and other related nematode species, opening great opportunities in the field of nematode transgenesis. Here we describe how these antibiotic selection systems can be easily combined with many well-established genetic approaches to study gene function, improving time- and cost-effectiveness of the nematode genetic toolbox.

Keywords: Antibiotic; C. elegans; Transformation marker; Transgenesis.

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