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. 1989 Dec;78(3):323-8.

Human monoclonal antibodies to HIV-1: cross-reactions with gag and env products

B J Pollock  1 , A S McKenzie, B E Kemp, D A McPhee, A J D'Apice
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Human monoclonal antibodies to HIV-1: cross-reactions with gag and env products

B J Pollock et al. Clin Exp Immunol. 1989 Dec.

Abstract

Human monoclonal antibodies were raised by in vitro immunization of normal human spleen cells with denatured HIV-1 and subsequent fusion to an Epstein-Barr virus (EBV) transformed cell line. Three monoclonals reacted with both gag-encoded p24 core protein and env-encoded gp41 transmembrane glycoprotein. The cross-reaction was confirmed by reactivity with p24- and gp41-derived recombinant peptides. The peptides share two amino acid sequences which may be the basis for the cross-reaction. Attempted epitope mapping using synthetic peptides was unsuccessful due to non-specific reactivity with the short linear peptides.

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