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. 2014 May 13;9(5):e97517.
doi: 10.1371/journal.pone.0097517. eCollection 2014.

First identification and characterization of porcine enterovirus G in the United States

Srivishnupriya Anbalagan  1 , Richard A Hesse  2 , Ben M Hause  1
Affiliations

First identification and characterization of porcine enterovirus G in the United States

Srivishnupriya Anbalagan et al. PLoS One. .

Abstract

Porcine enterovirus G (EV-G) is a member of the family Picornavirdae, genus Enterovirus. To date, eleven EV-G types (EV-G1 through EV-G11) have been identified in pigs from Asia and Europe however they have never been reported in North America. In this study, we isolated and characterized the complete genome of NP/2013/USA, an EV-G from a porcine diarrhea sample from the United States. The complete genome consists of 7,390 nucleotides excluding the 3' poly(A) tail, and has an open reading frame that encodes a 2,169 amino acid polyprotein. NP/2013/USA was most similar at the nucleotide (84%) and amino acid (95%) level to the HM131607, an EV-G1 type isolated from China in 2012.

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Conflict of interest statement

Competing Interests: The authors have the following interest. B.M.H. and S.A. are employed by Newport Laboratories, a for-profit organization that offers diagnostic testing and produces vaccines for the livestock industries, the funder for this study. There are no patents, products in development or marketed products to declare. This does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials, as detailed online in the guide for authors.

Figures

Figure 1
Figure 1. Phylogenetic tree of NP/2013/USA with Enterovirus G, Teschovirus and Sapelovirus genomes.
Maximum-likelihood analysis in combination with 1000 bootstrap replicates was used to derive trees based on the nucleotide sequence in the whole genome. A scale representing the number of nucleotide changes is shown. Bootstrap values are shown to the left of major nodes.
See this image and copyright information in PMC

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