Laboratory evaluation of the BD MAX MRSA assay

doi:10.1128/JCM.00237-14

Comparative Study

. 2014 Jul;52(7):2686-8.

doi: 10.1128/JCM.00237-14. Epub 2014 May 14.

Laboratory evaluation of the BD MAX MRSA assay

Raymond Widen¹, Vicki Healer², Suzane Silbert²

Affiliations

¹ Esoteric Testing Laboratory, Pathology Department, Tampa General Hospital, Tampa, Florida, USA rwiden@tgh.org.
² Esoteric Testing Laboratory, Pathology Department, Tampa General Hospital, Tampa, Florida, USA.

PMID: 24829235
PMCID: PMC4097742
DOI: 10.1128/JCM.00237-14

Comparative Study

Laboratory evaluation of the BD MAX MRSA assay

Raymond Widen et al. J Clin Microbiol. 2014 Jul.

. 2014 Jul;52(7):2686-8.

doi: 10.1128/JCM.00237-14. Epub 2014 May 14.

Authors

Raymond Widen¹, Vicki Healer², Suzane Silbert²

Affiliations

¹ Esoteric Testing Laboratory, Pathology Department, Tampa General Hospital, Tampa, Florida, USA rwiden@tgh.org.
² Esoteric Testing Laboratory, Pathology Department, Tampa General Hospital, Tampa, Florida, USA.

PMID: 24829235
PMCID: PMC4097742
DOI: 10.1128/JCM.00237-14

Abstract

A comparison between the BD MAX MRSA and Xpert MRSA assays was performed using 239 nares samples. A 97.9% overall agreement between the two molecular assays was observed. The BD MAX MRSA assay proved to be a reliable alternative for a highly automated system to detect methicillin-resistant Staphylococcus aureus (MRSA) in patient nares samples.

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Figures

**FIG 1**
Comparison of hands-on times and walkaway times for the Xpert and BD MAX MRSA assays for 16 samples, displayed as hours:minutes:seconds.

**FIG 2**
Comparison of hands-on times and walkaway times for the Xpert and BD MAX MRSA assays for 24 samples, displayed as hours:minutes:seconds.

See this image and copyright information in PMC

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Rocchetti TT, Silbert S, Gostnell A, Kubasek C, Jerris R, Vong J, Widen R. Rocchetti TT, et al. Pract Lab Med. 2018 May 24;12:e00102. doi: 10.1016/j.plabm.2018.e00102. eCollection 2018 Nov. Pract Lab Med. 2018. PMID: 30009245 Free PMC article.
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Mortensen JE, Ventrola C, Hanna S, Walter A. Mortensen JE, et al. BMC Clin Pathol. 2015 May 28;15:9. doi: 10.1186/s12907-015-0010-8. eCollection 2015. BMC Clin Pathol. 2015. PMID: 26019686 Free PMC article.
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Dalpke AH, Hofko M, Stock C, Zimmermann S. Dalpke AH, et al. J Clin Microbiol. 2014 Dec;52(12):4343-6. doi: 10.1128/JCM.02306-14. Epub 2014 Sep 17. J Clin Microbiol. 2014. PMID: 25232162 Free PMC article.

References

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1. Drews SJ, Willey BM, Kreiswirth N, Wang M, Ianes T, Mitchell J, Latchford M, McGeer AJ, Katz KC. 2006. Verification of the IDI-MRSA assay for detecting methicillin-resistant Staphylococcus aureus in diverse specimen types in a core laboratory setting. J. Clin. Microbiol. 44:3794–3796. 10.1128/JCM.01509-06 - DOI - PMC - PubMed
1. Donnio PY, Fevrier F, Bifani P, Dehem M, Kervegant C, Wilhelm N, Lerestif ALG, Lafforgue N, Cormier M, the MR-MSSA Study Group of the College de Bacteriologie-Virologie-Hygeine des Hopitaux de France. Le Coustumier A. 2007. Molecular and epidemiologic evidence for spread of multiresistant methicillin-susceptible Staphylococcus aureus strains in hospitals. Antimicrob. Agents Chemother. 51:4342–4350. 10.1128/AAC.01414-06 - DOI - PMC - PubMed
1. Klein E, Smith DL, Laxminarayan R. 2007. Hospitalizations and deaths caused by methicillin-resistant Staphylococcus aureus, United States, 1999–2005. Emerg. Infect. Dis. 13:1840–1846. 10.3201/eid1312.070629 - DOI - PMC - PubMed
1. Peterson LR, Hacek DM, Robiscek A. 2007. Case study: an MRSA intervention at Evanston Northwestern Healthcare. Jt. Comm. J. Qual. Patient Saf. 33:732–738 - PubMed

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[1] Hardy K, Price C, Szczepura A, Gossain S, Davies R, Stallard N, Shabir S, McMurray C, Bradbury A, Hawkey PM. 2010. Reduction in the rate of methicillin-resistant Staphylococcus aureus acquisition in surgical wards by rapid screening for colonization: a prospective, cross-over study. Clin. Microbiol. Infection. 16:333–339. 10.1111/j.1469-0691.2009.02899.x - DOI - PubMed

[2] Hardy K, Price C, Szczepura A, Gossain S, Davies R, Stallard N, Shabir S, McMurray C, Bradbury A, Hawkey PM. 2010. Reduction in the rate of methicillin-resistant Staphylococcus aureus acquisition in surgical wards by rapid screening for colonization: a prospective, cross-over study. Clin. Microbiol. Infection. 16:333–339. 10.1111/j.1469-0691.2009.02899.x - DOI - PubMed

[3] Drews SJ, Willey BM, Kreiswirth N, Wang M, Ianes T, Mitchell J, Latchford M, McGeer AJ, Katz KC. 2006. Verification of the IDI-MRSA assay for detecting methicillin-resistant Staphylococcus aureus in diverse specimen types in a core laboratory setting. J. Clin. Microbiol. 44:3794–3796. 10.1128/JCM.01509-06 - DOI - PMC - PubMed

[4] Drews SJ, Willey BM, Kreiswirth N, Wang M, Ianes T, Mitchell J, Latchford M, McGeer AJ, Katz KC. 2006. Verification of the IDI-MRSA assay for detecting methicillin-resistant Staphylococcus aureus in diverse specimen types in a core laboratory setting. J. Clin. Microbiol. 44:3794–3796. 10.1128/JCM.01509-06 - DOI - PMC - PubMed

[5] Donnio PY, Fevrier F, Bifani P, Dehem M, Kervegant C, Wilhelm N, Lerestif ALG, Lafforgue N, Cormier M, the MR-MSSA Study Group of the College de Bacteriologie-Virologie-Hygeine des Hopitaux de France. Le Coustumier A. 2007. Molecular and epidemiologic evidence for spread of multiresistant methicillin-susceptible Staphylococcus aureus strains in hospitals. Antimicrob. Agents Chemother. 51:4342–4350. 10.1128/AAC.01414-06 - DOI - PMC - PubMed

[6] Donnio PY, Fevrier F, Bifani P, Dehem M, Kervegant C, Wilhelm N, Lerestif ALG, Lafforgue N, Cormier M, the MR-MSSA Study Group of the College de Bacteriologie-Virologie-Hygeine des Hopitaux de France. Le Coustumier A. 2007. Molecular and epidemiologic evidence for spread of multiresistant methicillin-susceptible Staphylococcus aureus strains in hospitals. Antimicrob. Agents Chemother. 51:4342–4350. 10.1128/AAC.01414-06 - DOI - PMC - PubMed

[7] Klein E, Smith DL, Laxminarayan R. 2007. Hospitalizations and deaths caused by methicillin-resistant Staphylococcus aureus, United States, 1999–2005. Emerg. Infect. Dis. 13:1840–1846. 10.3201/eid1312.070629 - DOI - PMC - PubMed

[8] Klein E, Smith DL, Laxminarayan R. 2007. Hospitalizations and deaths caused by methicillin-resistant Staphylococcus aureus, United States, 1999–2005. Emerg. Infect. Dis. 13:1840–1846. 10.3201/eid1312.070629 - DOI - PMC - PubMed

[9] Peterson LR, Hacek DM, Robiscek A. 2007. Case study: an MRSA intervention at Evanston Northwestern Healthcare. Jt. Comm. J. Qual. Patient Saf. 33:732–738 - PubMed

[10] Peterson LR, Hacek DM, Robiscek A. 2007. Case study: an MRSA intervention at Evanston Northwestern Healthcare. Jt. Comm. J. Qual. Patient Saf. 33:732–738 - PubMed

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Laboratory evaluation of the BD MAX MRSA assay

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Laboratory evaluation of the BD MAX MRSA assay

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