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. 1978 Feb;5(2):435-50.
doi: 10.1093/nar/5.2.435.

Overmethylation of DNAs by the EcoRI methylase

K L Berkner, W R Folk
Free PMC article

Overmethylation of DNAs by the EcoRI methylase

K L Berkner et al. Nucleic Acids Res. 1978 Feb.
Free PMC article

Abstract

EcoRI methylase is able to catalyze methy incorporation into DNA at sequences other than the canonical EcoRI site. At high enzyme concentrations and over a wide range of pH and ionic strengths, EcoRI methylase modifies polyoma DNA (which contains one EcoRI site) at a number of sites. This modification prevents EcoRI endonuclease activity, and thus is presumably at or near the EcoRI sequences (5') NAATTN.

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