Effect of essential oil of Rosa Damascena on human colon cancer cell line SW742

Abstract

Aim: In this study, we report the effect of the essential oil of Rosa Damascena on human colon cancer cell line (SW742) and human fibroblast cells.

Background: Colon cancer is the second most common fatal malignancy. Owing to the existence of many side effects and problems related to common treatments such as surgery, chemotherapy and radiotherapy, alternative treatments are being investigated. Some herbal medicines have shown promising results against different types of cancers. Herbal medicines used have included the use naturally occurring essential oils.

Patients and methods: The essential oil of Rosa Damascena was obtained by distillation and its effect on SW742 cell-line and fibroblast cells were investigated with cell culture. The cells were cultured and different volumes of essential oil were induced to the cells. After48hincubation, cell survival was measured and using statistical analysis, the findings were evaluated and reported.

Results: This study showed that soluble part of Rosa Damascena oil increases cell proliferation in high volumes and the non-soluble component decreases cell proliferation.

Conclusion: The effects of essential oils, such as Rosa Damascena, on cell proliferation require more thorough investigation.

Keywords: Cell survival; Colon cancer cell line (SW742); Essential oil; Human fibroblast cell; Rosa Damascena.

Figure 1

Fibroblast and SW742 cells morphology in the presence of A) 0µl and B) 5µl of essential oil.

Figure 2

Fibroblast (left) and SW742 (right) cells morphology in the absenceof essential oil (the cells are cultured in the separated plate).

Figure 3

SW742 cell survival in the presence of different volumes of herbalessential oil after 48h incubation time using MTT assay.Cells populations that are cultured in another plate in the absence of essential oil are used as outer control.All amounts are different from control (P value ≤ 0.001).

Figure 4

Fibroblast cells survival in the presence of different volumes of herbal essential oil after 48h incubation time using MTT assay. Population of the cells that are cultured in anotherplate in the absence of essential oil is used as outer control. All amounts are different from control (P value ≤ 0.001, except for 10μl ≤ 0.01).

Figure 5

SW742 cell survival in the presence of different volumes of herbal essential oil after 48h incubation time using MTT assay. Inner control is thecells that are not treated with essential oil but are cultured in the same plate. The values of the (0μl), (1, 2 and 3μl), (4μl), (5μl) and (10μl) sets are statistically different.

Figure 6

Fibroblast cell survival in the presence of different volumes of herbal essential oil after 48h incubation time using MTT assay.Inner control is thecells that are not treated with essential oil but are cultured in the same dish. The values of the (0μl), (1, 2, 3 and 4μl), (5μl) and (10μl) sets are statistically different.