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. 1989 Jul-Sep:21:451-8.
doi: 10.1007/BF02917288.

Transformation of human kidney epithelial cells to tumorigenicity by nickel(II) and V-HA-RAS oncogene

A Haugen  1 , D Ryberg, I L Hansteen, H Dalen
Affiliations

Transformation of human kidney epithelial cells to tumorigenicity by nickel(II) and V-HA-RAS oncogene

A Haugen et al. Biol Trace Elem Res. 1989 Jul-Sep.

Abstract

Nickel is a toxic metal of environmental concern that has been found to be carcinogenic in man and animals. Primary human kidney (NHKE) cells were immortalized or rescued from senescence after exposure to NiSO4. The cell lines (IHKE) displayed abnormal karyotype and anchorage independent growth was observed. However, none of the IHKE cells produced tumor upon injection into athymic nude mice. Transfer of the v-Ha-ras oncogene into IHKE cells induced conversion of the immortalized cells into cell lines (THKE) that were tumorigenic when transplanted into athymic nude mice. Ha-ras DNA was present in the transformed cell lines and expressed at high level.

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References

    1. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1373-6 - PubMed
    1. Cancer Res. 1978 Jun;38(6):1479-96 - PubMed
    1. J Mol Biol. 1975 Nov 5;98(3):503-17 - PubMed
    1. Proc Natl Acad Sci U S A. 1981 Jan;78(1):313-7 - PubMed
    1. Nature. 1983 Aug 18-24;304(5927):596-602 - PubMed

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