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. 2014 Oct;159(10):2605-14.
doi: 10.1007/s00705-014-2116-1. Epub 2014 May 21.

Immune and inflammatory response in pigs during acute influenza caused by H1N1 swine influenza virus

Małgorzata Pomorska-Mól  1 , Iwona Markowska-Daniel, Krzysztof Kwit, Ewelina Czyżewska, Arkadiusz Dors, Jarosław Rachubik, Zygmunt Pejsak
Affiliations

Immune and inflammatory response in pigs during acute influenza caused by H1N1 swine influenza virus

Małgorzata Pomorska-Mól et al. Arch Virol. 2014 Oct.

Abstract

Swine influenza (SI) is an acute respiratory disease of pigs, caused by swine influenza virus (SIV). Little is known about the inflammatory response in the lung during acute SI and its correlation with clinical signs or lung pathology. Moreover, until now there has been a limited amount of data available on the relationship between the concentrations of pro- and anti-inflammatory cytokines in the lungs and the serum concentration of acute-phase proteins (APPs) in SIV-infected pigs. In the present study, the porcine inflammatory and immune responses during acute influenza caused by H1N1 SIV (SwH1N1) were studied. Nine pigs were infected intratracheally, and five served as controls. Antibodies against SIV were measured by haemagglutination inhibition assay, and the influenza-virus-specific T-cell response was measured using a proliferation assay. C-reactive protein (CRP), haptoglobin (Hp), serum amyloid A (SAA), and pig major acute-phase protein (Pig-MAP) the concentrations in serum and concentration of IL-1β, IL-6, IL-8, IL-10, TNF-α and IFN-γ in lung tissues were measured using commercial ELISAs.

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Figures

Fig. 1
Fig. 1
Individual rectal temperatures of pigs inoculated intratracheally with swine influenza virus (H1N1)
Fig. 2
Fig. 2
Changes observed at 4 and 7 days post-inoculation (dpi) in the lungs of pigs infected intratracheally with swine influenza virus (H1N1) and in control pigs
Fig. 3
Fig. 3
Mean (±SD) stimulation index values in pigs inoculated intratracheally with H1N1 swine influenza virus and in control pigs. The bold line indicates the value considered to represent antigen-specific proliferation
Fig. 4
Fig. 4
Concentrations of CRP, Hp, SAA and Pig-MAP in serum of pigs before and at various time points after intratracheal infection with H1N1swine influenza virus (mean ± SD). *p < 0.05, significant increase compared to control animals
Fig. 5
Fig. 5
Quantification of cytokines in lungs of control pigs and pigs with acute influenza caused by H1N1 swine influenza virus (mean ± SD). R, right; L, left; *, significant difference compared to the corresponding tissue sample from control pigs
See this image and copyright information in PMC

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