Different antiviral activity and cell specificity of interferon preparations produced by mouse peritoneal cells at 37 degrees C and at 26 degrees C

Abstract

Three sublines of mouse L cells and mouse embryo fibroblasts were used for determination of the antiviral activity of mouse interferons produced by nonadherent peritoneal exudate cells incubated either at 37 degrees C or at 26 degrees C. IFN produced at 37 degrees C or at 26 degrees C had the same antiviral activity in L Borgen, L929 cells. However, in MEC IFN-37 degrees had relatively higher activity than IFN-26 degrees. Of the interferon investigated only IFN-37 degrees exhibited antiviral activity in the established line of rat kidney cells. The IFN preparations showed no activity in the human and chicken cells. The studies on the sensitivity of viruses to both forms of IFN revealed that EMC and VSV viruses were equally sensitive to IFN-26 degrees C. However, the replication of EMC virus was more strongly inhibited by IFN-37 degrees than the multiplication of VSV virus.