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Case Reports
. 2014 Aug;52(8):3114-7.
doi: 10.1128/JCM.00615-14. Epub 2014 May 21.

Development of oxacillin resistance in a patient with recurrent Staphylococcus aureus bacteremia

Eileen M Burd  1 , Mohammad Tauqeer Alam  2 , Karla D Passalacqua  2 , Ameeta S Kalokhe  2 , Molly E Eaton  2 , Sarah W Satola  3 , Colleen S Kraft  3 , Timothy D Read  2
Affiliations
Case Reports

Development of oxacillin resistance in a patient with recurrent Staphylococcus aureus bacteremia

Eileen M Burd et al. J Clin Microbiol. 2014 Aug.

Abstract

Whole-genome sequencing was used to compare longitudinal isolates of Staphylococcus aureus that developed resistance to oxacillin (MIC up to 16 μg/ml). The mecA gene was absent. A novel 5-bp TATCC frameshift insertion in a gene encoding an ABC transporter similar to that of the teichoic acid translocation ATP-binding protein TagH and a 3-bp GCT nonframeshift insertion in the pdhA pyruvate dehydrogenase gene were detected in the oxacillin-resistant isolates.

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Figures

FIG 1
FIG 1
Partial protein sequence alignment showing truncated ABC transporter sequence in isolates 2 and 3. The wild-type sequence, as in the N315 reference sequence, and the isolate 1 sequence is 505 amino acids long, whereas in oxacillin-resistant isolates 2 and 3, it is truncated at amino acid position 87 due to a frameshift insertion mutation.
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References

    1. Limbago B, Fosheim GE, Schoonover V, Crane CE, Nadle J, Petit S, Heltzel D, Ray SM, Harrison LH, Lynfield R, Dumyati G, Townes JM, Schaffner W, Mu Y, Fridkin SK. 2009. Characterization of methicillin-resistant Staphylococcus aureus isolates collected in 2005 and 2006 from patients with invasive disease: a population-based analysis. J. Clin. Microbiol. 47:1344–1351. 10.1128/JCM.02264-08 - DOI - PMC - PubMed
    1. Inouye M, Conway TC, Zobel J, Holt KE. 2012. Short read sequence typing (SRST): multi-locus sequence types from short reads. BMC Genomics 13:338–338. 10.1186/1471-2164-13-338 - DOI - PMC - PubMed
    1. Schmieder R, Edwards R. 2011. Quality control and preprocessing of metagenomic datasets. Bioinformatics 27:863–864. 10.1093/bioinformatics/btr026 - DOI - PMC - PubMed
    1. Li H, Durbin R. 2010. Fast and accurate long-read alignment with Burrows-Wheeler transform. Bioinformatics 26:589–595. 10.1093/bioinformatics/btp698 - DOI - PMC - PubMed
    1. McKenna A, Hanna M, Banks E, Sivachenko A, Cibulskis K, Kernytsky A, Garimella K, Altshuler D, Gabriel S, Daly M, DePristo MA. 2010. The Genome Analysis Toolkit: a MapReduce framework for analyzing next-generation DNA sequencing data. Genome Res. 20:1297–1303. 10.1101/gr.107524.110 - DOI - PMC - PubMed

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