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. 2014 Aug 25;471(1-2):197-205.
doi: 10.1016/j.ijpharm.2014.05.020. Epub 2014 May 20.

Improved gene transfer with histidine-functionalized mesoporous silica nanoparticles

David Brevet  1 , Ouahiba Hocine  1 , Anthony Delalande  2 , Laurence Raehm  1 , Clarence Charnay  1 , Patrick Midoux  2 , Jean-Olivier Durand  3 , Chantal Pichon  4
Affiliations

Improved gene transfer with histidine-functionalized mesoporous silica nanoparticles

David Brevet et al. Int J Pharm. .

Abstract

Mesoporous silica nanoparticles (MSN) were functionalized with aminopropyltriethoxysilane (MSN-NH2) then L-histidine (MSN-His) for pDNA delivery in cells and in vivo. The complexation of pDNA with MSN-NH2 and MSN-His was first studied with gel shift assay. pDNA complexed with MSN-His was better protected from DNase degradation than with MSN-NH2. An improvement of the transfection efficiency in cells was observed with MSN-His/pDNA compared to MSN-NH2/pDNA, which could be explained by a better internalization of MSN-His. The improvement of the transfection efficiency with MSN-His was also observed for gene transfer in Achilles tendons in vivo.

Keywords: Histidine; Mesoporous silica nanoparticles; Nucleic acid delivery; Transfection in vivo.

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