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. 2014 Feb 28:(2):e1073.
doi: 10.3897/BDJ.2.e1073. eCollection 2014.

DNA barcoding reveals a new record of Potamogeton distinctus (Potamogetonaceae) and its natural hybrids, P. distinctus x P. nodosus and P. distinctus x P. wrightii ) (P. x malainoides) from Myanmar

Yu Ito  1 , Norio Tanaka  2 , Rachun Pooma  3 , Nobuyuki Tanaka  4
Affiliations

DNA barcoding reveals a new record of Potamogeton distinctus (Potamogetonaceae) and its natural hybrids, P. distinctus x P. nodosus and P. distinctus x P. wrightii ) (P. x malainoides) from Myanmar

Yu Ito et al. Biodivers Data J. .

Abstract

Indo-China floristic region is among the 34 richest floristic regions of the world, and its plant diversity is still under investigation. Here we report a new record of an aquatic plant, Potamogetondistinctus, from Myanmar, a part of the region, that is detected by means of DNA barcoding method. The molecular method further identified the other specimens as hybrids of Potamogeton: one is Potamogeton×malainoides (Potamogetondistinctus × Potamogetonwrightii), and the other is Potamogetondistinctus × Potamogetonnodosus. The first of these was thus far genetically confirmed in China, but the parental combination of the hybrid in Myanmar was reciprocal to those reported from China. The second hybrid was also recorded from China, but the maternal lineage was revealed for the first time, in this case it was Potamogetondistinctus. The present study showed that 1) nrITS is useful to distinguish closely related Potamogeton species as well as hybrids among them and 2) atpB-rbcL has higher utility than other frequently used plastid DNA markers. We thus propose nrITS and atpB-rbcL as DNA barcoding markers for future Potamogeton studies.

Keywords: DNA barcoding; Myanmar; Potamogeton; flora; new record.

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Figures

Figure 1.
Figure 1.
The most parsimonious trees of Potamogeton based on (A) the combined plastid DNA (atpB–rbcL, rpl20–rps12, trnT–trnL, trnL, trnL–trnF) sequences and (B) nrITS sequences. Each one of the two outgroups is trimmed to clarify ingroup phylogeny. ACCTRAN optimisation is used for branch length measures; terminals are aligned with dotted lines. Numbers above the branches indicate bootstrap support (BP) calculated in the maximum parsimony, and those below indicate Bayesian posterior probabilities (PP). Samples in regular and bold face indicate comparative ones and those from Myanmar, respectively. Some accessions in each tree represent multiple identical accessions. Note that some samples have heterogeneous nrITS copies; for these, the sequence pairs are named #1 and #2, respectively, and colored in red.
Figure 2.
Figure 2.
A voucher specimen of Potamogeton distinctus (N. Tanaka & al. 080061).
Figure 3.
Figure 3.
A voucher specimen of Potamogeton distinctus (N. Tanaka & al. 080657).
Figure 4.
Figure 4.
A voucher specimen of Potamogeton ×malainoides (N. Tanaka & al. 080631).
Figure 5.
Figure 5.
A voucher specimen of Potamogeton distinctus × Potamogeton nodosus (N. Tanaka & al. 080662).
See this image and copyright information in PMC

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