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. 2014 May 5;15(5):7639-50.
doi: 10.3390/ijms15057639.

Optimization of cell adhesion on mg based implant materials by pre-incubation under cell culture conditions

Regine Willumeit  1 , Anneke Möhring  2 , Frank Feyerabend  3
Affiliations

Optimization of cell adhesion on mg based implant materials by pre-incubation under cell culture conditions

Regine Willumeit et al. Int J Mol Sci. .

Abstract

Magnesium based implants could revolutionize applications where orthopedic implants such as nails, screws or bone plates are used because they are load bearing and degrade over time. This prevents a second surgery to remove conventional implants. To improve the biocompatibility we studied here if and for how long a pre-incubation of the material under cell culture conditions is favorable for cell attachment and proliferation. For two materials, Mg and Mg10Gd1Nd, we could show that 6 h pre-incubation are already enough to form a natural protective layer suitable for cell culture.

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Figures

Figure 1.
Figure 1.
(A) Corrosion rate; (B) pH values and (C) osmolality determined after the indicated pre-incubation time.
Figure 2.
Figure 2.
Life Dead Staining of Saos-2 cells incubated on two materials with varying pre-incubation times.
Figure 3.
Figure 3.
SEM images of Saos-2 cells incubated on two materials with varying pre-incubation times.
Figure 4.
Figure 4.
EDX element mapping of specimens with Saos-2 cells incubated on two materials with varying pre-incubation times.
See this image and copyright information in PMC

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