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. 2014 Aug:103:25-8.
doi: 10.1016/j.mimet.2014.04.014. Epub 2014 May 21.

Clinical evaluation of a disposable amperometric magneto-genosensor for the detection and identification of Streptococcus pneumoniae

Alma Sotillo  1 , María Pedrero  2 , Manuela de Pablos  3 , José Luis García  4 , Ernesto García  5 , Pedro García  5 , José Manuel Pingarrón  2 , Jesús Mingorance  6 , Susana Campuzano  7
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Free article

Clinical evaluation of a disposable amperometric magneto-genosensor for the detection and identification of Streptococcus pneumoniae

Alma Sotillo et al. J Microbiol Methods. 2014 Aug.
Free article

Abstract

A disposable PCR-based amperometric magneto-genosensor for detection and identification of Streptococcus pneumoniae was evaluated. ROC curve analysis used to determine optimal signal cutoff values yielded a sensitivity of 91% and a specificity of 90%. The method was also tested for the direct detection of pneumococci in clinical samples.

Keywords: Biosensors; Streptococcus mitis group; Streptococcus pneumoniae; lytA gene.

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