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. 2014 Aug:103:3-5.
doi: 10.1016/j.mimet.2014.05.006. Epub 2014 May 21.

Taxonomic database and cut-off value for processing mcrA gene 454 pyrosequencing data by MOTHUR

Sizhong Yang  1 , Susanne Liebner  2 , Mashal Alawi  2 , Oliver Ebenhöh  2 , Dirk Wagner  2
Affiliations

Taxonomic database and cut-off value for processing mcrA gene 454 pyrosequencing data by MOTHUR

Sizhong Yang et al. J Microbiol Methods. 2014 Aug.

Abstract

The functional mcrA gene of methanogens can generate phylogeny as congruent as the 16S rRNA gene phylogeny. For the mcrA sequences amplified by mlas/mcrA-rev primers, we created a database for taxonomical classification and propose cut-off values for OTU clustering and further analysis of α- and β-diversity with the MOTHUR software.

Keywords: Cut-off; MOTHUR; Methanogens; Taxonomic database; mcrA gene.

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