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. 2014 Aug;58(8):4928-30.
doi: 10.1128/AAC.02394-14. Epub 2014 May 27.

Association between pncA gene mutations, pyrazinamidase activity, and pyrazinamide susceptibility testing in Mycobacterium tuberculosis

Akio Aono  1 , Kinuyo Chikamatsu  2 , Hiroyuki Yamada  2 , Tomoko Kato  3 , Satoshi Mitarai  3
Affiliations

Association between pncA gene mutations, pyrazinamidase activity, and pyrazinamide susceptibility testing in Mycobacterium tuberculosis

Akio Aono et al. Antimicrob Agents Chemother. 2014 Aug.

Abstract

We determined MICs for, confirmed the presence of pncA mutations in, and performed pyrazinamidase testing on colonies (subclones) obtained from seven isolates that exhibited differential pyrazinamide (PZA) susceptibility. Six of the seven strains were found to exhibit characteristics resulting from the mixture of strains possessing different properties. In addition, our analysis revealed large pncA-spanning deletions (1,565 bp, 4,475 bp, and 6,258 bp) in three strains that showed high PZA resistance.

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Figures

FIG 1
FIG 1
Flow diagram of PZA susceptibility analysis, pncA mutation testing, PZase analysis, and PZA MIC determinations (see also Fig. S1 and Table S2 in the supplemental material).
See this image and copyright information in PMC

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