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. 2014;22(2):75-7.
doi: 10.1590/1413-78522014220200798.

A manual method to obtain platelet rich plasma

Fabiana Paulino Marques  1 , Sheila Jean McNeill Ingham  2 , Andrea Forgas  1 , Carlos Eduardo da Silveira Franciozi  3 , Pedro Henrique Sasaki  2 , Rene Jorge Abdalla  2
Affiliations

A manual method to obtain platelet rich plasma

Fabiana Paulino Marques et al. Acta Ortop Bras. 2014.

Abstract

Objective: This study is to report a manual method to obtain platelet rich plasma (PRP).

Methods: For this study 61 ml of peripheral blood was obtained and submitted to centrifugation at 541g for 5 min. The centrifugation separates the blood into three components: red blood cells, buffy coat and platelet rich plasma. Blood and platelet rich plasma samples were sent to the Hospital's Laboratory and platelets and leukocytes were measured.

Results: A sample of 637 blood donors was evaluated. The platelet yield efficiency was 86.77% and the increase in platelet concentration factor was 2.89 times. The increase in leukocyte concentration factor was 1.97 times.

Conclusion: The method described here produces leukocyte-rich and platelet-rich plasma with a high platelet and leukocyte increased factor. Level of Evidence IV, Controlled Laboratory Study.

Keywords: Blood platelets; Methods.; Platelet-rich plasma.

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Figures

Figure 1
Figure 1. Platelet poor plasma (right) and platelet rich plasma (left).
Figure 2
Figure 2. Platelet rich plasma clot activated with autologous thrombin and 10% calcium gluconate.
Figure 3
Figure 3. Examples of clinical application of PRP: A) Placement of PRP in autograft to be used for reconstruction of the anterior cruciate ligament; B) Soaked autograft wrapped in gauze soaked with PRP. C) Application of PRP after knee arthroscopy.
See this image and copyright information in PMC

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