Comment on

• Mitotic chromosomes are chromatin networks without a mechanically contiguous protein scaffold.

  Poirier MG, Marko JF. Poirier MG, et al. Proc Natl Acad Sci U S A. 2002 Nov 26;99(24):15393-7. doi: 10.1073/pnas.232442599. Epub 2002 Nov 18. Proc Natl Acad Sci U S A. 2002. PMID: 12438695 Free PMC article.
• The three-dimensional structure of in vitro reconstituted Xenopus laevis chromosomes by EM tomography.

  König P, Braunfeld MB, Sedat JW, Agard DA. König P, et al. Chromosoma. 2007 Aug;116(4):349-72. doi: 10.1007/s00412-007-0101-0. Epub 2007 Feb 28. Chromosoma. 2007. PMID: 17333236
• Analysis of cryo-electron microscopy images does not support the existence of 30-nm chromatin fibers in mitotic chromosomes in situ.

  Eltsov M, Maclellan KM, Maeshima K, Frangakis AS, Dubochet J. Eltsov M, et al. Proc Natl Acad Sci U S A. 2008 Dec 16;105(50):19732-7. doi: 10.1073/pnas.0810057105. Epub 2008 Dec 8. Proc Natl Acad Sci U S A. 2008. PMID: 19064912 Free PMC article.
• Human mitotic chromosomes consist predominantly of irregularly folded nucleosome fibres without a 30-nm chromatin structure.

  Nishino Y, Eltsov M, Joti Y, Ito K, Takata H, Takahashi Y, Hihara S, Frangakis AS, Imamoto N, Ishikawa T, Maeshima K. Nishino Y, et al. EMBO J. 2012 Apr 4;31(7):1644-53. doi: 10.1038/emboj.2012.35. Epub 2012 Feb 17. EMBO J. 2012. PMID: 22343941 Free PMC article.