Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014 Jun;51(6):1161-6.
doi: 10.1007/s13197-011-0609-4. Epub 2011 Dec 24.

Microbial based assay for specific detection of β-lactam group of antibiotics in milk

Sougata Das  1 , Naresh Kumar  1 , Raghu Hirikyathanahalli Vishweswaraiah  1 , Lopamudra Haldar  2 , Manju Gaare  1 , Vinai Kumar Singh  1 , Anil Kumar Puniya  1
Affiliations

Microbial based assay for specific detection of β-lactam group of antibiotics in milk

Sougata Das et al. J Food Sci Technol. 2014 Jun.

Abstract

The spore forming Bacillus cereus (66) was screened for the induction of β-lactamase in presence of an inducer using iodometric assay. A significant induction in marker enzyme was observed in B. cereus 66 at maximum residual limit (MRL) of penicillin, ampicillin, cloxacillin, amoxicillin, cefalexin, and cephazolin belonging to β-lactam group of antibiotics. A microbial based assay, where enzyme induction was optimized at pH 7.0, temperature 30°C, and whey powder (0.25%) after 4 h of incubation. The spore based assay was tested with milk samples spiked with 6 different β-lactam antibiotics. The results were 100 and 83.33% in correlation with microbial receptor and inhibition based assay, respectively. Overall, spore based assay can be a useful and cost effective tool for the specific detection of β-lactam group of antibiotics in milk.

Keywords: Assay; Bacillus; Induction; Spore; β-lactam; β-lactamase.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Disc assay result on comparative inhibitory effect of antibiotic on B.cereus 66; A = Minimum Inhibitory concentration and B = European Union/ Codex Alimentarius Commission Maximum Residual Limit (EU/Codex MRL) (n = 3)
See this image and copyright information in PMC

References

    1. Ahmad S, Yadava JNS. Rapid detection of β-lactam antibiotic resistance among clinical isolates of Escherichia.coli. Indian Vet Med J. 1979;32:256–259.
    1. Aureli P, Ferrini AM, Mannoni V. Presumptive identification of sulphonamide and antibiotic residues in milk by microbial inhibitor tests. Food Control. 1996;7(3):165–168. doi: 10.1016/0956-7135(96)00025-4. - DOI
    1. Catlin BW. Iodometric detection of Haemophiluc influenzae β–lactamase: rapid presumptive test for Ampicillin resistance. Antimicrob Agents Chemother. 1975;7:265–270. doi: 10.1128/AAC.7.3.265. - DOI - PMC - PubMed
    1. Chen Y, Succi J, Tenover FC, Koehler TM. β-lactamase genes of the penicillin-susceptible bacillus anthracis sterne strain. J Bacteriol. 2003;185(3):823–830. doi: 10.1128/JB.185.3.823-830.2003. - DOI - PMC - PubMed
    1. Davis BR, Abraham EP, Melling J. Seperation, purification and properties of β-Lactamases-I and β-Lactamases-II from Bacillus cereus 569/H/9. Biochem J. 1974;143:115–127. - PMC - PubMed

LinkOut - more resources