Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014:2014:687936.
doi: 10.1155/2014/687936. Epub 2014 Apr 27.

Morphogenesis of Mammary Glands in Buffalo (Bubalus bubalis)

Amit Challana  1 , Anuradha Gupta  1 , Neelam Bansal  1 , Varinder Uppal  1
Affiliations

Morphogenesis of Mammary Glands in Buffalo (Bubalus bubalis)

Amit Challana et al. Anat Res Int. 2014.

Abstract

The present research was elucidated on the morphogenesis of mammary gland of buffalo during prenatal development. Total of 16 foetuses ranging from 1.2 cm (34 days) to 108 cm CVRL (curved crown rump length) (317 days) were used for study. The study revealed that mammary line was first observed at 1.2 cm CVRL (34 days), mammary hillock at 1.7 cm (37 days), and mammary bud at 2.6 cm CVRL (41 days) foetuses. Epidermal cone was found at 6.7 cm CVRL (58 days) whereas primary and secondary ducts were observed at 7.4 cm CVRL (62 days) and 15 cm CVRL (96 days), respectively. Connective tissue whorls were reported at 18.2 cm CVRL (110 days) and internal elastic lamina and muscle layers at 24.1 cm CVRL (129 days). Lobules were observed at 29.3 cm CVRL (140 days), rosette of furstenberg at 39.5 cm CVRL (163 days), and keratin plug at 45.5 cm CVRL (176 days) foetus. Primordia of sweat and sebaceous glands around hair follicle were seen at 21.2 cm CVRL (122 days) of foetal life. Differentiation of all the skin layers along with cornification was observed at 69 cm (229 days) in group III foetuses.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Photomicrograph of mammary line (ML) of buffalo foetuses in the ventral region at 1.2 cm CVRL (34 days). Hematoxylin and Eosin stain ×100.
Figure 2
Figure 2
Photomicrograph of mammary hillock (MH) of buffalo foetuses at 1.7 cm CVRL (37 days). Hematoxylin and Eosin stain ×100.
Figure 3
Figure 3
Photomicrograph of mammary bud of buffalo foetuses showing concentric layers of mesenchymal cells and blood vessel (BV) lined by basement membrane (BM) at 2.6 cm CVRL (41 days). Hematoxylin and Eosin stain ×100.
Figure 4
Figure 4
Photomicrograph of primary sprout (PS) and epidermal cone (EC) at 6.7 cm CVRL (58 days). Hematoxylin and Eosin stain ×40.
Figure 5
Figure 5
Photomicrograph of initiation of teat development along with epidermis, dermis, gland sinus, and hair follicle (HF) at 7.4 cm CVRL (62 days). Hematoxylin and Eosin stain ×40.
Figure 6
Figure 6
Photomicrograph of luminized primary sprout (PS) surrounded by connective tissue (CT) at 12.6 cm CVRL (85 days). Hematoxylin and Eosin stain ×400.
Figure 7
Figure 7
Photomicrograph of secondary sprout (SS) at 15 cm CVRL (96 days). Hematoxylin and Eosin stain ×400.
Figure 8
Figure 8
Photomicrograph connective tissue whorl (CWr) at 18.2 cm CVRL (110 days). Hematoxylin and Eosin stain ×100.
Figure 9
Figure 9
Photomicrograph of rosette of furstenberg (RF) at 39.5 cm CVRL (163 days). Hematoxylin and Eosin stain ×100.
Figure 10
Figure 10
Photomicrograph of gland sinus (GS) surrounded by blood vessels (BV) at 47.8 cm CVRL (181 days). Hematoxylin and Eosin stain ×100.
Figure 11
Figure 11
Photomicrograph of keratin plug at tip of teat in 45.5 cm CVRL (176 days). Hematoxylin and Eosin stain ×40.
Figure 12
Figure 12
Photomicrograph of hair follicle surrounded by sebaceous gland and sweat glands at 82 cm CVRL (259 days). Hematoxylin and Eosin stain ×100.
See this image and copyright information in PMC

References

    1. Chaurasia D. Histochemical and Ultrastructural studies on mammary gland in buffalo (Bubalusbubalis) [Ph.D. thesis] Nagpur, India: Maharashtra Animal and Fishery Sciences University; 2009.
    1. Huynh HT, Robitaille G, Turner JD. Establishment of bovine mammary epithelial cells (MAC-T): an in vitro model for bovine lactation. Experimental Cell Research. 1991;197(2):191–199. - PubMed
    1. Lopez AC. Characterization of Differentiation of Bovine Mammary Epithelial Cells [Honours Scholar Thesis] University of Connecticut; 2011.
    1. Taneja VK, Birthal PS. Role of buffalo in food security in Asia. Proceedings of 4th Asian Buffalo Congress; 2003; New Delhi, India. pp. 1–12.
    1. Ahlawat SPS, Vij PK, Tantia MS. Conservation of buffalo genetic resources. Proceedings of 4th Asian Buffalo Congress; 2003; New Delhi, India. pp. 62–68.

LinkOut - more resources