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. 2014 Aug;32(8):819-21.
doi: 10.1038/nbt.2925. Epub 2014 Jun 1.

Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system

Mehdi Ghorbal  1 , Molly Gorman  2 , Cameron Ross Macpherson  2 , Rafael Miyazawa Martins  2 , Artur Scherf  2 , Jose-Juan Lopez-Rubio  1
Affiliations

Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system

Mehdi Ghorbal et al. Nat Biotechnol. 2014 Aug.

Abstract

Genome manipulation in the malaria parasite Plasmodium falciparum remains largely intractable and improved genomic tools are needed to further understand pathogenesis and drug resistance. We demonstrated the CRISPR-Cas9 system for use in P. falciparum by disrupting chromosomal loci and generating marker-free, single-nucleotide substitutions with high efficiency. Additionally, an artemisinin-resistant strain was generated by introducing a previously implicated polymorphism, thus illustrating the value of efficient genome editing in malaria research.

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References

    1. Mol Biochem Parasitol. 2011 May;177(1):29-34 - PubMed
    1. Nat Methods. 2011 Oct 23;8(12):1078-82 - PubMed
    1. Nucleic Acids Res. 2012 Jul;40(12):5313-31 - PubMed
    1. Nature. 2014 Jan 2;505(7481):50-5 - PubMed
    1. Mol Microbiol. 2003 Jul;49(1):179-91 - PubMed

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