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. 2014:2014:916531.
doi: 10.1155/2014/916531. Epub 2014 May 5.

An Immunomodulatory Protein (Ling Zhi-8) from a Ganoderma lucidum Induced Acceleration of Wound Healing in Rat Liver Tissues after Monopolar Electrosurgery

Hao-Jan Lin  1 , Yushan-Sophie Chang  2 , Li-Hsiang Lin  3 , Chiung-Fang Haung  4 , Chia-Yu Wu  5 , Keng-Liang Ou  6
Affiliations

An Immunomodulatory Protein (Ling Zhi-8) from a Ganoderma lucidum Induced Acceleration of Wound Healing in Rat Liver Tissues after Monopolar Electrosurgery

Hao-Jan Lin et al. Evid Based Complement Alternat Med. 2014.

Abstract

The purpose of this study was to investigate the effect of an immunomodulatory protein (Ling Zhi-8, LZ-8) on wound healing in rat liver tissues after monopolar electrosurgery. Animals were sacrificed for evaluations at 0, 3, 7, and 28 days postoperatively. It was found that the wound with the LZ-8 treatment significantly increases wound healing. Western blot analysis clearly indicated that the expression of NF-κB was decreased at 3, 7, and 28 days when liver tissues were treated with LZ-8. Moreover, caspase-3 activity of the liver tissue also significantly decreases at 7 and 28 days, respectively. DAPI staining and TUNEL assays revealed that only a minimal dispersion of NF-κB was found on the liver tissue treated with LZ-8 at day 7 as compared with day 3 and tissues without LZ-8 treatment. Similarly, apoptosis was decreased on liver tissues treated with LZ-8 at 7 days when compared to the control (monopolar electrosurgery) tissues. Therefore, the analytical results demonstrated that LZ-8 induced acceleration of wound healing in rat liver tissues after monopolar electrosurgery.

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Figures

Figure 1
Figure 1
The wound healing effects on wounds in rat liver tissues after monopolar electrosurgery for different healing days (H&E staining): (a) SS group and (b) SS + LZ-8 group.
Figure 2
Figure 2
The wound healing effects on wounds in rat liver tissues after monopolar electrosurgery for 0 days (Masson's trichrome staining): (a) wound area, (b) the site of thermal injury area and injury area, and (c) percentage of each area. Data are expressed as the means ± SEM (n = 5). No significant difference in the thermal injury area, injury area, and wound area at day 0.
Figure 3
Figure 3
The wound healing effects on wounds in rat liver tissues after monopolar electrosurgery for 3 days (Masson's trichrome staining): (a) wound area, (b) the site of thermal injury area, injury area, and normal area, and (c) percentage of each area. Data are expressed as the means ± SEM (n = 5).  *P < 0.05,  **P < 0.01,  ***P < 0.001 compared with SS group.
Figure 4
Figure 4
The wound healing effects on wounds in rat liver tissues after monopolar electrosurgery for 7 days (Masson's trichrome staining): (a) wound area, (b) the site of apoptosis area, fibrosis area, and normal area, and (c) percentage of each area. Data are expressed as the means ± SEM (n = 5).  *P < 0.05,  **P < 0.01,  ***P < 0.001 compared with SS group.
Figure 5
Figure 5
The wound healing effects on wounds in rat liver tissues after monopolar electrosurgery for 28 days (Masson's trichrome staining): (a) wound area, (b) the site of apoptosis area, fibrosis area, and proliferate area, and (c) percentage of each area. Data are expressed as the means ± SEM (n = 5).  *P < 0.05,  **P < 0.01,  ***P < 0.001 compared with SS group.
Figure 6
Figure 6
The Western blot assay results from rats liver treated with SS group and SS + LZ-8 group after different healing days: (a) expressions of NF-κB and (b) expressions of caspase-3. Data are expressed as the means ± SEM (n = 5).  *P < 0.05,  **P < 0.01,  ***P < 0.001 compared with SS group.
Figure 7
Figure 7
The immunofluorescence staining results from rats liver treated with SS group and SS + LZ-8 group after different healing days.
Figure 8
Figure 8
The TUNEL staining assay results from rats liver treated with SS group and SS + LZ-8 group after 7 days of healing.
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