Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014:2014:916531.
doi: 10.1155/2014/916531. Epub 2014 May 5.

An Immunomodulatory Protein (Ling Zhi-8) from a Ganoderma lucidum Induced Acceleration of Wound Healing in Rat Liver Tissues after Monopolar Electrosurgery

Hao-Jan Lin  1 , Yushan-Sophie Chang  2 , Li-Hsiang Lin  3 , Chiung-Fang Haung  4 , Chia-Yu Wu  5 , Keng-Liang Ou  6
Affiliations

An Immunomodulatory Protein (Ling Zhi-8) from a Ganoderma lucidum Induced Acceleration of Wound Healing in Rat Liver Tissues after Monopolar Electrosurgery

Hao-Jan Lin et al. Evid Based Complement Alternat Med. 2014.

Abstract

The purpose of this study was to investigate the effect of an immunomodulatory protein (Ling Zhi-8, LZ-8) on wound healing in rat liver tissues after monopolar electrosurgery. Animals were sacrificed for evaluations at 0, 3, 7, and 28 days postoperatively. It was found that the wound with the LZ-8 treatment significantly increases wound healing. Western blot analysis clearly indicated that the expression of NF-κB was decreased at 3, 7, and 28 days when liver tissues were treated with LZ-8. Moreover, caspase-3 activity of the liver tissue also significantly decreases at 7 and 28 days, respectively. DAPI staining and TUNEL assays revealed that only a minimal dispersion of NF-κB was found on the liver tissue treated with LZ-8 at day 7 as compared with day 3 and tissues without LZ-8 treatment. Similarly, apoptosis was decreased on liver tissues treated with LZ-8 at 7 days when compared to the control (monopolar electrosurgery) tissues. Therefore, the analytical results demonstrated that LZ-8 induced acceleration of wound healing in rat liver tissues after monopolar electrosurgery.

PubMed Disclaimer

Figures

Figure 1
Figure 1
The wound healing effects on wounds in rat liver tissues after monopolar electrosurgery for different healing days (H&E staining): (a) SS group and (b) SS + LZ-8 group.
Figure 2
Figure 2
The wound healing effects on wounds in rat liver tissues after monopolar electrosurgery for 0 days (Masson's trichrome staining): (a) wound area, (b) the site of thermal injury area and injury area, and (c) percentage of each area. Data are expressed as the means ± SEM (n = 5). No significant difference in the thermal injury area, injury area, and wound area at day 0.
Figure 3
Figure 3
The wound healing effects on wounds in rat liver tissues after monopolar electrosurgery for 3 days (Masson's trichrome staining): (a) wound area, (b) the site of thermal injury area, injury area, and normal area, and (c) percentage of each area. Data are expressed as the means ± SEM (n = 5).  *P < 0.05,  **P < 0.01,  ***P < 0.001 compared with SS group.
Figure 4
Figure 4
The wound healing effects on wounds in rat liver tissues after monopolar electrosurgery for 7 days (Masson's trichrome staining): (a) wound area, (b) the site of apoptosis area, fibrosis area, and normal area, and (c) percentage of each area. Data are expressed as the means ± SEM (n = 5).  *P < 0.05,  **P < 0.01,  ***P < 0.001 compared with SS group.
Figure 5
Figure 5
The wound healing effects on wounds in rat liver tissues after monopolar electrosurgery for 28 days (Masson's trichrome staining): (a) wound area, (b) the site of apoptosis area, fibrosis area, and proliferate area, and (c) percentage of each area. Data are expressed as the means ± SEM (n = 5).  *P < 0.05,  **P < 0.01,  ***P < 0.001 compared with SS group.
Figure 6
Figure 6
The Western blot assay results from rats liver treated with SS group and SS + LZ-8 group after different healing days: (a) expressions of NF-κB and (b) expressions of caspase-3. Data are expressed as the means ± SEM (n = 5).  *P < 0.05,  **P < 0.01,  ***P < 0.001 compared with SS group.
Figure 7
Figure 7
The immunofluorescence staining results from rats liver treated with SS group and SS + LZ-8 group after different healing days.
Figure 8
Figure 8
The TUNEL staining assay results from rats liver treated with SS group and SS + LZ-8 group after 7 days of healing.
See this image and copyright information in PMC

References

    1. Liboon J, Funkhouser W, Terris DJ. A comparison of mucosal incisions made by scalpel, CO2 laser, electrocautery, and constant-voltage electrocautery. Otolaryngology—Head and Neck Surgery. 1997;116(3):379–385. - PubMed
    1. Carew JF, Ward RF, LaBruna A, Torzilli PA, Schley WS. Effects of scalpel, electrocautery, and CO2 and KTP lasers on wound healing in rat tongues. The Laryngoscope. 1998;108(3):373–380. - PubMed
    1. Pearce JA. Electrosurgery. New York, NY, USA: John Wiley & Sons; 1986.
    1. Maness WL, Rober FW, Clark RE, Cataldo E, Haddad AW. Tissue damage from electrosurgical power output variations in hamster tongues. The Journal of Prosthetic Dentistry. 1979;42(4):456–460. - PubMed
    1. Maness WL, Roeber FW, Clark RE, Cataldo E, Riis D, Haddad AW. Histologic evaluation of electrosurgery with varying frequency and waveform. The Journal of Prosthetic Dentistry. 1978;40(3):304–308. - PubMed

LinkOut - more resources