Genome instability in blood cells of a BRCA1+ breast cancer family

Abstract

Background: BRCA1 plays an essential role in maintaining genome stability. Inherited BRCA1 germline mutation (BRCA1+) is a determined genetic predisposition leading to high risk of breast cancer. While BRCA1+ induces breast cancer by causing genome instability, most of the knowledge is known about somatic genome instability in breast cancer cells but not germline genome instability.

Methods: Using the exome-sequencing method, we analyzed the genomes of blood cells in a typical BRCA1+ breast cancer family with an exon 13-duplicated founder mutation, including six breast cancer-affected and two breast cancer unaffected members.

Results: We identified 23 deleterious mutations in the breast cancer-affected family members, which are absent in the unaffected members. Multiple mutations damaged functionally important and breast cancer-related genes, including transcriptional factor BPTF and FOXP1, ubiquitin ligase CUL4B, phosphorylase kinase PHKG2, and nuclear receptor activator SRA1. Analysis of the mutations between the mothers and daughters shows that most mutations were germline mutation inherited from the ancestor(s) while only a few were somatic mutation generated de novo.

Conclusion: Our study indicates that BRCA1+ can cause genome instability with both germline and somatic mutations in non-breast cells.

Figure 1

Pedigree of the BRCA1+ family used in the study. Eight members of this family were sequenced by exome sequencing, of which #1 (62y), #2 (53y), #4 (35y), #5 (35y), #6 (36y), #7 (35y) are breast cancer-affected and #3 (65y) and #8 (45y) are breast cancer-unaffected members. All six affected members are BRCA1+ whereas the two unaffected members are not. #9 and #10 were used in validation to remove the variants transmitted from them to their daughters.