Pentoxifylline attenuates nitrogen mustard-induced acute lung injury, oxidative stress and inflammation
- PMID: 24886962
- PMCID: PMC4271840
- DOI: 10.1016/j.yexmp.2014.05.009
Pentoxifylline attenuates nitrogen mustard-induced acute lung injury, oxidative stress and inflammation
Abstract
Nitrogen mustard (NM) is a toxic alkylating agent that causes damage to the respiratory tract. Evidence suggests that macrophages and inflammatory mediators including tumor necrosis factor (TNF)α contribute to pulmonary injury. Pentoxifylline is a TNFα inhibitor known to suppress inflammation. In these studies, we analyzed the ability of pentoxifylline to mitigate NM-induced lung injury and inflammation. Exposure of male Wistar rats (150-174 g; 8-10 weeks) to NM (0.125 mg/kg, i.t.) resulted in severe histopathological changes in the lung within 3d of exposure, along with increases in bronchoalveolar lavage (BAL) cell number and protein, indicating inflammation and alveolar-epithelial barrier dysfunction. This was associated with increases in oxidative stress proteins including lipocalin (Lcn)2 and heme oxygenase (HO)-1 in the lung, along with pro-inflammatory/cytotoxic (COX-2(+) and MMP-9(+)), and anti-inflammatory/wound repair (CD163+ and Gal-3(+)) macrophages. Treatment of rats with pentoxifylline (46.7 mg/kg, i.p.) daily for 3d beginning 15 min after NM significantly reduced NM-induced lung injury, inflammation, and oxidative stress, as measured histologically and by decreases in BAL cell and protein content, and levels of HO-1 and Lcn2. Macrophages expressing COX-2 and MMP-9 also decreased after pentoxifylline, while CD163+ and Gal-3(+) macrophages increased. This was correlated with persistent upregulation of markers of wound repair including pro-surfactant protein-C and proliferating nuclear cell antigen by Type II cells. NM-induced lung injury and inflammation were associated with alterations in the elastic properties of the lung, however these were largely unaltered by pentoxifylline. These data suggest that pentoxifylline may be useful in treating acute lung injury, inflammation and oxidative stress induced by vesicants.
Keywords: Lung injury; Macrophages; Mustards; Oxidative stress; Pentoxifylline; Vesicants.
Copyright © 2014 Elsevier Inc. All rights reserved.
Conflict of interest statement
The authors do not have any conflict of interest.
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References
-
- Aikawa P, Zhang H, Barbas CS, Pazetti R, Correia C, Mauad T, Silva E, Sannomiya P, Poli-de-Figueiredo LF, Nakagawa NK. The effects of low and high tidal volume and pentoxifylline on intestinal blood flow and leukocyte-endothelial interactions in mechanically ventilated rats. Respir Care. 2011;56:1942–1949. - PubMed
-
- Almario B, Wu S, Peng J, Alapati D, Chen S, Sosenko IR. Pentoxifylline and prevention of hyperoxia-induced lung -injury in neonatal rats. Pediatr Res. 2012;71:583–589. - PubMed
-
- Anderson DR, Taylor SL, Fetterer DP, Holmes WW. Evaluation of protease inhibitors and an antioxidant for treatment of sulfur mustard-induced toxic lung injury. Toxicology. 2009;263:41–46. - PubMed
-
- Barkhordari K, Karimi A, Shafiee A, Soltaninia H, Khatami MR, Abbasi K, Yousefshahi F, Haghighat B, Brown V. Effect of pentoxifylline on preventing acute kidney injury after cardiac surgery by measuring urinary neutrophil gelatinase - associated lipocalin. J Cardiothorac Surg. 2011;6:8. - PMC - PubMed
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