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. 1989 Aug;11(4):217-25.
doi: 10.1016/0141-8130(89)90072-x.

Hydrophobic cluster analysis of the primary sequences of alpha-amylases

E Raimbaud  1 , A Buleon, S Perez, B Henrissat
Affiliations

Hydrophobic cluster analysis of the primary sequences of alpha-amylases

E Raimbaud et al. Int J Biol Macromol. 1989 Aug.

Abstract

The amino acid sequences of 18 alpha-amylases have been compared by hydrophobic cluster analysis. The method was first calibrated with two alpha-amylases (Aspergillus oryzae and pig pancreas) whose three-dimensional structures are known. It was then applied to the other alpha-amylases resulting in straightforward sequence alignments which could be used for structure prediction. It was found that all alpha-amylases which were investigated display the same basic super-secondary structure with a (beta alpha)8 barrel. Most of the secondary structure elements of the protein cores could be assigned to segments of the amino acid sequences. In addition, six sub-families could be identified, based upon specific similarities occurring in the variable regions of alpha-amylases.

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