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. 2014 Sep;54(3):315-22.
doi: 10.1007/s12088-014-0446-z. Epub 2014 Jan 29.

Characterization and Fungal Inhibition Activity of Siderophore from Wheat Rhizosphere Associated Acinetobacter calcoaceticus Strain HIRFA32

D V Maindad  1 , V M Kasture  2 , H Chaudhari  1 , D D Dhavale  2 , B A Chopade  3 , D P Sachdev  1
Affiliations

Characterization and Fungal Inhibition Activity of Siderophore from Wheat Rhizosphere Associated Acinetobacter calcoaceticus Strain HIRFA32

D V Maindad et al. Indian J Microbiol. 2014 Sep.

Abstract

Acinetobacter calcoaceticus HIRFA32 from wheat rhizosphere produced catecholate type of siderophore with optimum siderophore (ca. 92 % siderophore units) in succinic acid medium without FeSO4 at 28 °C and 24 h of incubation. HPLC purified siderophore appeared as pale yellow crystals with molecular weight [M(+1)] m/z 347.18 estimated by LCMS. The structure elucidated by (1)H NMR, (13)C NMR, HMQC, HMBC, NOESY and decoupling studies, revealed that siderophore composed of 2,3-dihydroxybenzoic acid with hydroxyhistamine and threonine as amino acid subunits. In vitro study demonstrated siderophore mediated mycelium growth inhibition (ca. 46.87 ± 0.5 %) of Fusarium oxysporum. This study accounts to first report on biosynthesis of acinetobactin-like siderophore by the rhizospheric strain of A. calcoaceticus and its significance in inhibition of F. oxysporum.

Keywords: Acinetobacter calcoaceticus; Acinetobactin-like siderophore; Fusarium oxysporum; NMR; Wheat rhizosphere.

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Figures

Fig. 1
Fig. 1
Representative 1H NMR spectrum of siderophore from A. calcoaceticus HIRFA32 1H NMR spectrum was recorded on D2O (300 MHz). Scale represents δ values
Fig. 2
Fig. 2
a Representative image depicting siderophore mediated inhibition of F. oxysporum by A. calcoaceticus HIRFA32 under iron limiting conditions. A Restricted growth of F. oxysporum; B Growth of F. oxysporum in absence of A. calcoaceticus HIRFA32 (Control), b Growth inhibition of Fusarium oxysporum by partially purified siderophore from A. calcoaceticus HIRFA32. A 20 μl of partially purified siderophore from A. calcoaceticus HIRFA32; B 10 fold diluted partially purified siderophore; C 20 μl of supernatant of 96 h grown A. calcoaceticus HIRFA32 in iron restricted media; D Solvent blank
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References

    1. Huddedar SB, Shete AM, Tilekar JN, Gore SD, Dhavale DD, Chopade BA. Isolation, characterization and plasmid pUPI126 mediated indole 3 acetic acid (IAA) production in Acinetobacter strains from rhizosphere of wheat. Appl Biochem Biotechnol. 2002;102:21–29. doi: 10.1385/ABAB:102-103:1-6:021. - DOI - PubMed
    1. Rokhbakhsh-Zamin F, Sachdev D, Kazemi-Pour N, Engineer A, Pardesi KR, Zinjarde SS, Dhakephalkar PK, Chopade BA. Characterization of plant growth promoting traits of Acinetobacter species isolated from rhizosphere of Pennisetum glaucum. J Microbiol Biotech. 2011;21:556–566. - PubMed
    1. Sachdev D, Nema P, Dhakephalkar P, Zinjarde S, Chopade B. Assessment of 16S rRNA gene based phylogenetic diversity of Acinetobacter community from the rhizosphere of wheat. Microbiol Res. 2010;165:627–638. doi: 10.1016/j.micres.2009.12.002. - DOI - PubMed
    1. Sorensen J, Van Elsas JD, Trevors JT, Wellington EMH. Modern soil microbiology. New York: Marcel Dekker; 1997. The rhizosphere as a habitat for soil microorganisms; pp. 21–45.
    1. Bais HP, Park SW, Weir TL, Callaway RM, Vivanco JM. How plants communicate using the underground information superhighway. Trends Plant Sci. 2004;9:26–32. doi: 10.1016/j.tplants.2003.11.008. - DOI - PubMed

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