Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2012 Sep 30;43(3):221-5.
eCollection 2012 Jul.

Plasma cortisol activity in rats under conditions of chronic stress supplemented with resveratrol

Miryam Vélez-Marín  1 , Alejandro Hurtado Salazar  2 , Luis F Uribe-Velásquez  3
Affiliations

Plasma cortisol activity in rats under conditions of chronic stress supplemented with resveratrol

Miryam Vélez-Marín et al. Colomb Med (Cali). .

Abstract
in English, Spanish

Objective: To determine the activity of cortisol in rats treated with exogenous adrenocorticotropic hormone (ACTH) and a resveratrol supplement.

Methods: Forty-eight adult female rats and 16 male rats of the strain (Rattus norvegicus) that were three months old and with body weights ranging from 200 to 250 g for females and 300 to 350 g for males were used and kept in controlled environmental conditions: temperature of 20±2° C and light-dark cycles of 14 and 10 hours. They were fed a balanced diet and had free access to water. The rats were randomly divided into four groups: group 1 - was treated with 5 µg/kg of ACTH i.p. every twelve hours; group 2 - received the same treatment with ACTH plus a grape extract supplement (resveratrol) of 40 mg/kg; group 3 - only received grape extract (resveratrol); and group 4 - received a saline solution (0.9%) i.p. and oral, and served as controls. The experimental design was a 2×2 factorial with two levels ACTH and two polyphenol levels (grape extract).

Results: No significant differences were found in blood cortisol concentrations, by day and gender, or by treatment effects (0.75 µg/dL ± 0.11; p <0.001).

Conclusion: Results suggest that chronic stress and consumption of resveratrol did not directly alter levels of plasmatic cortisol in either stressed or unstressed rats. It was concluded that the given dosage levels of ACTH possibly did not produce sufficient stimulation of the adrenal gland for these animals.

Objetivo: Determinar la actividad de cortisol en ratas tratadas con hormona adrecorticotropa (ACTH) exógena y un suplemento de resveratrol.

Metodos: Se utilizaron 48 ratas hembras adultas y 16 machos de la cepa wistar (Rattus norvegicus) de tres meses de edad y con un peso corporal de 200-250 g y 300-350 g para hembras y machos, respectivamente, que permanecieron en condiciones ambientales controladas, temperatura 20±2° C de ciclos de luz-oscuridad de 14 y 10 horas, respectivamente. Se les proporcionó alimento balanceado con libre acceso a agua. Las ratas fueron divididas en cuatro grupos al azar así: grupo 1, fue tratado con 5 µg/kg de ACTH i.p. cada 12 horas; grupo 2, recibió el mismo tratamiento con ACTH además de una suplementación oral de 40 mg/kg de extracto deshidratado de uva (resveratrol); grupo 3, solo recibió extracto de uva y el grupo 4, recibió solución salina y sirvió como control y (0.9%) i.p. y oral. El diseño experimental fue en factorial 2×2, con dos niveles de ACTH y dos niveles de polifenol.

Resultados: No se encontraron diferencias significativas del cortisol sanguíneo, con respecto al día y sexo, entre los tratamientos (0.75 µg/dL ± 0.11; p <0.001).

Conclusión: Los resultados sugieren que el estrés crónico y el consumo de resveratrol no alteran directamente los niveles plasmáticos de cortisol, en ratas estresadas y no estresadas. De la misma manera que, posiblemente la dosis utilizada de ACTH no produjo estimulación de la glándula suprarrenal en las ratas.

Keywords: Adrenocorticotropic hormone; antioxidants; phenolic compounds.

PubMed Disclaimer

Conflict of interest statement

Conflicts of interest : We declare no conflict of interest existed with the Universidad de Caldas who sponsored the research.

Similar articles

See all similar articles

Cited by

References

    1. Selye H. The evolution of the stress concept. Am Sci. 1973;61(6):692–699. - PubMed
    1. Nadal RAA. Mecanismos de susceptibilidad al estrés. Hipertensión. 2010;27:117–124.
    1. Vélez MM, Uribe VLF, Biosalud. Cómo afecta el estrés calórico la reproducción? (2) 2010;9:83–95.
    1. Miller DB, O'Callaghan JP Neuroendocrine aspects of the response to stress. Metabolism. 2002;51(6 ) 1:5–10. - PubMed
    1. Fauvel JP, Quelin P, Ducher M, Rakotomalala H, Laville M. Perceived job stress but not individual cardiovascular reactivity to stress is related to higher blood pressure at work. Hypertension. 2001;38(1):71–75. - PubMed