Identification of different phenotypes of interstitial cells in the upper and deep lamina propria of the human bladder dome
- PMID: 24893312
- DOI: 10.1016/j.juro.2014.05.096
Identification of different phenotypes of interstitial cells in the upper and deep lamina propria of the human bladder dome
Abstract
Purpose: There is increasing evidence for an important role of the lamina propria in bladder physiology and interstitial cells seem to have a key role in this area. Interstitial cells in the upper lamina propria have been studied most frequently. We characterized interstitial cells in the deeper lamina propria and hypothesized that the 2 interstitial cell populations have different phenotypes based on their ultrastructural and immunohistochemical properties.
Materials and methods: Tissue samples were obtained from macroscopically and microscopically normal areas of radical cystectomy specimens. A panel of immunohistochemical markers was used to characterize lamina propria interstitial cells. Single/double immunohistochemistry/immunofluorescence was performed. At a second phase electron microscopy was used to compare upper and deeper lamina propria interstitial cells.
Results: Overall the phenotype of upper lamina propria interstitial cells was vimentin, α-smooth muscle actin, caveolin-1 and 2, PDGFRα, and nonphosphorylated and phosphorylated connexin 43 positive, and CD34 and c-kit negative. The overall phenotype of deeper lamina propria interstitial cells was vimentin, CD34 and nonphosphorylated connexin 43 positive, and α-smooth actin, caveolin-1 and 2, PDGFRα, phosphorylated connexin 43 and c-kit negative. Based on ultrastructural findings upper lamina propria interstitial cells were fibroblasts with myoid features and sparse myofibroblasts while deeper lamina propria interstitial cells were interstitial cell of Cajal-like cells.
Conclusions: To our knowledge this is the first study of 2 main interstitial cell populations in the upper and deeper lamina propria of the human bladder with distinct ultrastructural and immunohistochemical phenotypes. Future research is needed to elucidate whether these morphological findings reflect different roles for upper and deeper lamina propria interstitial cells in bladder physiology.
Keywords: fibroblasts; interstitial cells of Cajal; mucous membrane; phenotype; urinary bladder.
Copyright © 2014 American Urological Association Education and Research, Inc. Published by Elsevier Inc. All rights reserved.
Similar articles
-
Morphological expression of KIT positive interstitial cells of Cajal in human bladder.J Urol. 2010 Jul;184(1):370-7. doi: 10.1016/j.juro.2010.03.005. Epub 2010 May 20. J Urol. 2010. PMID: 20488490 Free PMC article.
-
Interstitial cells in the urinary bladder--localization and function.Neurourol Urodyn. 2010;29(1):82-7. doi: 10.1002/nau.20739. Neurourol Urodyn. 2010. PMID: 20025023 Review.
-
3D-electron microscopic characterization of interstitial cells in the human bladder upper lamina propria.Neurourol Urodyn. 2018 Jan;37(1):89-98. doi: 10.1002/nau.23270. Epub 2017 Mar 31. Neurourol Urodyn. 2018. PMID: 28370277
-
Identification of PDGFRα positive populations of interstitial cells in human and guinea pig bladders.J Urol. 2012 Aug;188(2):639-47. doi: 10.1016/j.juro.2012.03.117. Epub 2012 Jun 15. J Urol. 2012. PMID: 22704452
-
The Mystery of the Interstitial Cells in the Urinary Bladder.Annu Rev Pharmacol Toxicol. 2018 Jan 6;58:603-623. doi: 10.1146/annurev-pharmtox-010617-052615. Epub 2017 Oct 6. Annu Rev Pharmacol Toxicol. 2018. PMID: 28992432 Review.
Cited by
-
Differentiated PDGFRα-Positive Cells: A Novel In-Vitro Model for Functional Studies of Neuronal Nitric Oxide Synthase.Int J Mol Sci. 2021 Mar 29;22(7):3514. doi: 10.3390/ijms22073514. Int J Mol Sci. 2021. PMID: 33805311 Free PMC article.
-
Studies of ultrastructure, gene expression, and marker analysis reveal that mouse bladder PDGFRA+ interstitial cells are fibroblasts.Am J Physiol Renal Physiol. 2022 Sep 1;323(3):F299-F321. doi: 10.1152/ajprenal.00135.2022. Epub 2022 Jul 14. Am J Physiol Renal Physiol. 2022. PMID: 35834272 Free PMC article.
-
The Urothelium: Life in a Liquid Environment.Physiol Rev. 2020 Oct 1;100(4):1621-1705. doi: 10.1152/physrev.00041.2019. Epub 2020 Mar 19. Physiol Rev. 2020. PMID: 32191559 Free PMC article. Review.
-
Minimal Invasive Cystometry and Intra-Abdominal Pressure Assessments in Rodents: A Novel Animal Study.Med Sci Monit. 2017 May 24;23:2500-2507. doi: 10.12659/msm.904760. Med Sci Monit. 2017. PMID: 28538709 Free PMC article.
-
Proliferation of Interstitial Cells in the Cyclophosphamide-Induced Cystitis and the Preventive Effect of Imatinib.Biomed Res Int. 2017;2017:3457093. doi: 10.1155/2017/3457093. Epub 2017 Jun 18. Biomed Res Int. 2017. PMID: 28698872 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical