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. 2014:2014:534821.
doi: 10.1155/2014/534821. Epub 2014 May 7.

Network of microRNAs-mRNAs interactions in pancreatic cancer

Elnaz Naderi  1 , Mehdi Mostafaei  2 , Akram Pourshams  1 , Ashraf Mohamadkhani  1
Affiliations

Network of microRNAs-mRNAs interactions in pancreatic cancer

Elnaz Naderi et al. Biomed Res Int. 2014.

Abstract

Background: MicroRNAs are small RNA molecules that regulate the expression of certain genes through interaction with mRNA targets and are mainly involved in human cancer. This study was conducted to make the network of miRNAs-mRNAs interactions in pancreatic cancer as the fourth leading cause of cancer death.

Methods: 56 miRNAs that were exclusively expressed and 1176 genes that were downregulated or silenced in pancreas cancer were extracted from beforehand investigations. MiRNA-mRNA interactions data analysis and related networks were explored using MAGIA tool and Cytoscape 3 software. Functional annotations of candidate genes in pancreatic cancer were identified by DAVID annotation tool.

Results: This network is made of 217 nodes for mRNA, 15 nodes for miRNA, and 241 edges that show 241 regulations between 15 miRNAs and 217 target genes. The miR-24 was the most significantly powerful miRNA that regulated series of important genes. ACVR2B, GFRA1, and MTHFR were significant target genes were that downregulated.

Conclusion: Although the collected previous data seems to be a treasure trove, there was no study simultaneous to analysis of miRNAs and mRNAs interaction. Network of miRNA-mRNA interactions will help to corroborate experimental remarks and could be used to refine miRNA target predictions for developing new therapeutic approaches.

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Figures

Figure 1
Figure 1
Interaction network of mRNAs and upregulated miRNAs in pancreatic cancer based on Cytoscape 3 software. Green circles shower miRNAs and other circles offer for mRNAs that are existing in yellow, orange, brick red, and red based on the number of their regulating miRNAs. When the colors tend to be red from yellow, the numbers of interactions increase. Yellow color represents one interaction. It means mRNA is controlled and regulated by miRNA. Orange color represents two interactions; brick red color, three interactions; and red color, four interactions and regulate by four miRNAs. Length edges are log P value. (If you zoom in the figure, you will find more details regarding network.)
See this image and copyright information in PMC

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