Vertebrate female germline--the acquisition of femaleness

Abstract

The cellular and molecular characteristics of female germ cells have primarily been studied in the mammalian ovary. In most female mammals, all primordial germ cells (PGCs) develop into oocytes early during ovary formation, and germline stem cells are few in number or absent in postnatal ovaries (Lei L, Spradling AC. Female mice lack adult germ-line stem cells but sustain oogenesis using stable primordial follicles. Proc Natl Acad Sci USA 2013, 110:8585-8590). Research efforts in the field have largely focused on meiosis and follicular development, but a fundamental question regarding establishment of femaleness, which is very important to understand the 'female' germline, has not been discussed sufficiently. Recent work has revealed the presence of germline stem cells in the vertebrate ovary, using the teleost fish, medaka (Oryzias latipes) (Nakamura S, Kobayashi K, Nishimura T, Higashijima S, Tanaka, M. Identification of germline stem cells in the ovary of teleost medaka. Science 2010, 328:1561-1563). This discovery allows direct comparison between female and male germline stem cells and raises an interesting and heretofore unaddressed issue regarding femaleness of germline stem cells. In this article, the germ cell behavior in the ovaries of different species is reviewed and compared, the molecular mechanisms underlying the generation of female germ cells are discussed, and the relationship between female germ cells and the surrounding somatic cells is examined.