Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014 Apr;48(2):295-302.
doi: 10.1590/s0034-8910.2014048004719.

Trypanosoma cruzi strains from triatomine collected in Bahia and Rio Grande do Sul, Brazil

Aline Rimoldi Ribeiro  1 , Vagner José Mendonça  2 , Renata Tomé Alves  3 , Isabel Martinez  2 , Renato Freitas de Araújo  4 , Fernanda Mello  5 , João Aristeu da Rosa  2
Affiliations

Trypanosoma cruzi strains from triatomine collected in Bahia and Rio Grande do Sul, Brazil

Aline Rimoldi Ribeiro et al. Rev Saude Publica. 2014 Apr.

Abstract
in English, Portuguese

Objective: Collection of triatomines in domestic, peridomestic and sylvatic environments in states of Bahia and Rio Grande do Sul, Northeastern and Southern Brazil respectively, and isolation of Trypanosoma cruzi strains.

Methods: First, the captured triatomines were identified using insect identification keys, then their intestinal content was examined by abdominal compression, and the samples containing trypanosomatid forms were inoculated in LIT medium and Swiss mice.

Results: Six triatomine species were collected in cities in Bahia, namely Panstrongylus geniculatus (01), Triatoma melanocephala (11), T. lenti (94), T. pseudomaculata (02), T. sherlocki (26) and T. sordida (460), and two in cities in Rio Grande do Sul, namely T. circummaculata (11) and T. rubrovaria (115). Out of the specimens examined, T. cruzi was isolated from 28 triatomine divided into four different species: T. melanocephala (one), T. lenti (one), T. rubrovaria (16) and T. sordida (10). Their index of natural infection by T. cruzi was 6.4%.

Conclusions: The isolation of T. cruzi strains from triatomines found in domestic and peridomestic areas shows the potential risk of transmission of Chagas disease in the studied cities. The maintenance of those T. cruzi strains in laboratory is intended to promote studies that facilitate the understanding of the parasite-vector-host relationship.

OBJETIVO: Isolar cepas de Trypanosoma cruzi em triatomíneos capturados nos ambientes domiciliar, peridomiciliar e silvestre da Bahia e do Rio Grande do Sul.

MÉTODOS: Os triatomíneos capturados nos estados da Bahia e Rio Grande do Sul foram identificados por meio de chaves entomológicas. O conteúdo intestinal foi examinado por compressão abdominal e as amostras que continham formas de Trypanosomatidae foram inoculadas em meio de cultura Liver Infusion Tryptose e em camundongos Swiss.

RESULTADOS: Foram identificadas seis espécies de triatomíneos nas coletas realizadas em municípios do Estado da Bahia: Panstrongylus geniculatus (01), Triatoma melanocephala (11), T. lenti (94), T. pseudomaculata (02), T. sherlocki (26) e T. sordida (460), e duas no Estado do Rio Grande do Sul: T. circummaculata (11) e T. rubrovaria (115). Dos exemplares examinados, T. cruzi foi isolado de 28 triatomíneos pertencentes a quatro espécies: T. melanocephala (uma), T. lenti (uma), T. rubrovaria (16) e T. sordida (10). O índice de infecção natural de triatomíneos por T. cruzi foi de 6,4%.

CONCLUSÕES: O isolamento de cepas de T. cruzi em triatomíneos encontrados no intra e peridomicílio reflete o potencial risco de transmissão da doença de Chagas nos municípios estudados.

PubMed Disclaimer

Conflict of interest statement

The authors declare that there is no conflict of interest.

Figures

Figure
Figure
Cities in Bahia and Rio Grande do Sul where triatomines were collected, 2002-2009.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Almeida CE, Vinhaes MC, Almeida JR, Silveira AC, Costa J. Monitoring the domiciliary and peridomiciliary invasion process of Triatoma rubrovaria in the State of Rio Grande do Sul, Brazil. Mem Inst Oswaldo Cruz. 2000;95(6):761–768. doi: 10.1590/S0074-02762000000600003. - DOI - PubMed
    1. Almeida CE, Pacheco RS, Haag K, Dupas S, Dotson EM, Costa J. Inferring from the Cyt B gene the Triatoma brasiliensis Neiva, 1911 (Hemiptera: Reduviidae: Triatominae) genetic structure and domiciliary infestation in the Sstate of Paraíba, Brazil. Am J Trop Med Hyg. 2008;78(5):791–802. - PubMed
    1. Araújo CAC, Waniek PJ, Jansen AM. An overview of Chagas disease and the role of triatomines on its distribution in Brazil. Vector Borne Zoonotic Dis. 2009;9(3):227–234. doi: 10.1089/vbz.2008.0185. - DOI - PubMed
    1. Bedin C, Mello F, Wilhelms TS, Torres MA, Estima C, Ferreira CF, et al. Vigilância ambiental: doença de Chagas no Rio Grande do Sul. Bol Epidemiol CEVS/RS. 2009;11(3):1–8.
    1. Chagas C. Nova tripanozomiaze humana: estudos sobre a morfolojia e o ciclo evolutivo do Schizotrypanum cruzi n.gen. n.sp., ajente etiolojico de nova entidade morbida do homem. Mem Inst Oswaldo Cruz. 1909;1(2):159–218. doi: 10.1590/S0074-02761909000200008. - DOI