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. 2014 Jun;48(2):91-7.
doi: 10.1007/s13139-013-0249-x. Epub 2014 Jan 25.

Expression patterns of glucose transporter-1 gene and thyroid specific genes in human papillary thyroid carcinoma

Sungeun Kim  1 , June-Key Chung  2 , Hae-Sook Min  3 , Joo-Hyun Kang  4 , Do Joon Park  5 , Jae Min Jeong  6 , Dong Soo Lee  7 , Sung-Hwae Park  3 , Bo Youn Cho  5 , Sinae Lee  8 , Myung Chul Lee  7
Affiliations

Expression patterns of glucose transporter-1 gene and thyroid specific genes in human papillary thyroid carcinoma

Sungeun Kim et al. Nucl Med Mol Imaging. 2014 Jun.

Abstract

Purpose: The expression of glucose transporter-1 (Glut-1) gene and those of major thyroid-specific genes were examined in papillary carcinoma tissues, and the expressions of these genes were compared with cancer differentiation grades.

Materials and methods: Twenty-four human papillary carcinoma tissues were included in this study. The expressions of Glut-1- and thyroid-specific genes [sodium/iodide symporter (NIS), thyroid peroxidase, thyroglobulin, TSH receptor and pendrin] were analyzed by RT-PCR. Expression levels were expressed as ratios versus the expression of beta-actin. Pathologic differentiation of papillary carcinoma was classified into a relatively well-differentiated group (n = 13) and relatively less differentiated group (n = 11).

Results: Glut-1 gene expression was significantly higher in the less differentiated group (0.66 ± 0.04) than in the well-differentiated group (0.59 ± 0.07). The expression levels of the NIS, PD and TG genes were significantly higher in the well-differentiated group (NIS: 0.67 ± 0.20, PD: 0.65 ± 0.21, TG: 0.74 ± 0.16) than in the less differentiated group (NIS: 0.36 ± 0.05, PD: 0.49 ± 0.08, TG: 0.60 ± 0.11), respectively. A significant negative correlation was found between Glut-1 and NIS expression, and positive correlations were found between NIS and TG, and between NIS and PD.

Conclusion: The NIS, PD and TG genes were highly expressed in well-differentiated thyroid carcinomas, whereas the Glut-1 gene was highly expressed in less differentiated thyroid carcinomas. These findings provide a molecular rationale for the management of papillary carcinoma, especially in the selection of FDG PET or radioiodine whole-body scan and I-131-based therapy.

Keywords: Glucose transporter-1; Papillary thyroid cancer; Pendrin; Sodium/iodide symporter; TSH receptor; Thyroglobulin; Thyroid peroxidase.

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Figures

Fig. 1
Fig. 1
Representative photomicrographs of relatively well-differentiated (a) and relatively less differentiated (b) papillary thyroid cancer (H&E, ×200). In relatively well-differentiated cancer, well-developed papillary structures with a fibrovascular core, typical nuclear features and scant cytoplasm were observed. In relatively less differentiated cancer, trabecular or tubular growth patterns with prominent tumor cell oxyphilic changes were observed
Fig. 2
Fig. 2
RT-PCR image of each gene in relatively well-differentiated (a) and relatively less differentiated (b) papillary carcinoma
Fig. 3
Fig. 3
Expression level of the NIS gene in relatively well-differentiated and relatively less differentiated papillary carcinoma
Fig. 4
Fig. 4
Expression level of the Glut-1 gene in relatively well-differentiated and relatively less differentiated papillary carcinoma
Fig. 5
Fig. 5
Correlations of the gene expressions of NIS and Glut-1 (a), NIS and TG (b), and NIS and PD (c)
See this image and copyright information in PMC

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