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. 2014;17(4):250-5.

Numerical status of CD4(+)CD25(+)FoxP3(+) and CD8(+)CD28(-) regulatory T cells in multiple sclerosis

Ebrahim Kouchaki  1 , Mahdi Salehi  2 , Mohammad Reza Sharif  3 , Hassan Nikoueinejad  4 , Hossein Akbari  5
Affiliations

Numerical status of CD4(+)CD25(+)FoxP3(+) and CD8(+)CD28(-) regulatory T cells in multiple sclerosis

Ebrahim Kouchaki et al. Iran J Basic Med Sci. 2014.

Abstract

Objectives: Regulatory T cells, including CD4+CD25+Fox3+ and CD8+CD28- cells play an important role in regulating the balance between immunity and tolerance. Since multiple sclerosis is an inflammatory autoimmune disease, regulatory T cells are considered to be involved in its pathogenesis. In this study, we investigated the circulatory numbers of the two mentioned types of regulatory T cells and also their association with different clinical characteristics in 84 multiple sclerosis patients.

Materials and methods: 84 patients with multiple sclerosis and 75 normal individuals were studied. Demographic and clinical information of all participants were collected via questionnaire and clinical examination as well as MRI. The peripheral blood frequency of two different subgroups of regulatory T cells (CD4+ CD25+Foxp3+ and CD8+CD28- cells) were analyzed by flow cytometry using anti-human antibodies conjugated with CD4-FITC / CD25-PE/Foxp3-PE-Cy5, CD3-PE/CD8a-PE-Cy5/CD28-FITC.

Results: The frequency of CD4+CD25+Foxp3+ cells in multiple sclerosis patients was significantly less than that in healthy controls (P=0.006) and in mild forms less than that in sever forms (P=0.003). There was not any correlation between the frequency of regulatory T cells and different clinical variables.

Conclusion: Our results showed that the number of CD4+CD25+Foxp3+ cells decreases significantly in multiple sclerosis patients, which probably shows the regulatory role of these cells in multiple sclerosis.

Keywords: CD4+CD25+Foxp3+ -; CD8+CD28- Regulatory T cells; Multiple sclerosis; Regulatory T cells.

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Figures

Figure 1
Figure 1
Flow cytometry results in a sample patient. A) Having gated on CD4+ cells, we counted CD4+CD25+Foxp3+ cells B) Having gated on CD3+ cells, we counted CD8+CD28- cells
Figure 2
Figure 2
Numbers of CD4+CD25+Foxp3+ cells and CD8+CD28-cells in peripheral blood samples of 84 MS patients and 75 healthy controls
See this image and copyright information in PMC

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