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. 2014:1170:425-45.
doi: 10.1007/978-1-4939-0888-2_23.

Detecting senescence: methods and approaches

Elizabeth P Crowe  1 , Timothy Nacarelli, Alessandro Bitto, Chad Lerner, Christian Sell, Claudio Torres
Affiliations

Detecting senescence: methods and approaches

Elizabeth P Crowe et al. Methods Mol Biol. 2014.

Abstract

The detection of senescent cells has become an important area of research in the aging field. Due to the complexity of the senescence program and the lack of a unique signature for senescence, the detection of these cells remains problematic. This is especially true for in vivo detection in aged or diseased tissue samples. This chapter outlines approaches for the detection of senescent cells based upon methods established for mesenchymal cells in culture. A stepwise approach to the detection of senescent cells using multiple techniques is provided.

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