Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014 Jun 10;14(6):10177-86.
doi: 10.3390/s140610177.

Quorum sensing activity in Pandoraea pnomenusa RB38

Robson Ee  1 , Yan-Lue Lim  2 , Lin-Xin Kin  3 , Wai-Fong Yin  4 , Kok-Gan Chan  5
Affiliations

Quorum sensing activity in Pandoraea pnomenusa RB38

Robson Ee et al. Sensors (Basel). .

Abstract

Strain RB38 was recovered from a former dumping area in Malaysia. MALDI-TOF mass spectrometry and genomic analysis identified strain RB-38 as Pandoraea pnomenusa. Various biosensors confirmed its quorum sensing properties. High resolution triple quadrupole liquid chromatography-mass spectrometry analysis was subsequently used to characterize the N-acyl homoserine lactone production profile of P. pnomenusa strain RB38, which validated that this isolate produced N-octanoyl homoserine lactone as a quorum sensing molecule. This is the first report of the production of N-octanoyl homoserine lactone by P. pnomenusa strain RB38.

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
Dendrogram of isolate RB38. MALDI-TOF analysis confirmed isolate RB38 clusters with Pandoraea pnomenusa.
Figure 2.
Figure 2.
Evolutionary tree of 16S rDNA nucleotide sequences of isolate RB-38 with its closest neighbours using the Neighbour-Joining method.
Figure 3.
Figure 3.
AHL screening of P. pnomenusa RB38. Biosensors C. violaceum CV026 was streaked adjacent to the test strains. Note that purple pigment shows quorum sensing activity. Erwinia carotovora GS101 (Positive control) and Erwinia carotovora PNP22 (Negative control) were included as controls.
Figure 4.
Figure 4.
Bioluminescence assay for production of AHLs by P. pnomenusa RB38. Each dot symbolizes the mean results of triplicate experiments. Negative control was included which is an extract from uninoculated LB broth.
Figure 5.
Figure 5.
Mass spectrometry analysis of AHLs produced by P. pnomenusa RB38. Mass spectrum shows that P. pnomenusa RB38 produced C8-HSL (a); Retention time of C8-HSL from spent supernatant of P. pnomenusa RB38 is the same as the retention time of synthetic C8-HSL (b).
See this image and copyright information in PMC

References

    1. Coenye T., Falsen E., Hoste B., Ohlen M., Goris J., Govan J.R., Gillis M., Vandamme P. Description of Pandoraea gen. nov. with Pandoraea apista sp. nov., Pandoraea pulmonicola sp. nov., Pandoraea pnomenusa sp. nov., Pandoraea sputorum sp. nov. and Pandoraea norimbergensis comb. nov. Int. J. Syst. Evol. Microbiol. 2000;50:887–899. - PubMed
    1. Daneshvar M.I., Hollis D.G., Steigerwalt A.G., Whitney A.M., Spangler L., Douglas M.P., Jordan J.G., MacGregor J.P., Hill B.C., Tenover F.C., et al. Assignment of CDC weak oxidizer group 2 (WO-2) to the genus Pandoraea and characterization of three new Pandoraea genomo species. J. Clin. Microbiol. 2001;39:1819–1826. - PMC - PubMed
    1. Coenye T., Liu L., Vandamme P., LiPuma J.J. Identification of Pandoraea species by 16S ribosomal DNA-based PCR assays. J. Clin. Microbiol. 2001;39:4452–4455. - PMC - PubMed
    1. Caraher E., Collins J., Herbert G., Murphy P.G., Gallagher C.G., Crowe M.J., Callaghan M., McClean S. Evaluation of in vitro virulence characteristics of the genus Pandoraea in lung epithelial cells. J. Med. Microbiol. 2008;57:15–20. - PubMed
    1. Aravena-Roman M. Cellular fatty acid-deficient Pandoraea isolated from a patient with cystic fibrosis. J. Med. Microbiol. 2008;57:252. - PubMed

Publication types

MeSH terms

LinkOut - more resources