Review of application of mass spectrometry for analyses of anterior eye proteome

Abstract

Proteins have important functional roles in the body, which can be altered in disease states. The eye is a complex organ rich in proteins; in particular, the anterior eye is very sophisticated in function and is most commonly involved in ophthalmic diseases. Proteomics, the large scale study of proteins, has greatly impacted our knowledge and understanding of gene function in the post-genomic period. The most significant breakthrough in proteomics has been mass spectrometric identification of proteins, which extends analysis far beyond the mere display of proteins that classical techniques provide. Mass spectrometry functions as a "mass analyzer" which simplifies the identification and quantification of proteins extracted from biological tissue. Mass spectrometric analysis of the anterior eye proteome provides a differential display for protein comparison of normal and diseased tissue. In this article we present the key proteomic findings in the recent literature related to the cornea, aqueous humor, trabecular meshwork, iris, ciliary body and lens. Through this we identified unique proteins specific to diseases related to the anterior eye.

Keywords: Glaucoma; Mass spectrometry; Ocular; Proteomics.

Figure 1

Comparing common protein functions in trabecular meshwork of eyes between fours students. 1: Anti-apoptosis; 2: Carbohydrate metabolic process; 3: Cell adhesion; 4: Cell cycle; 5: Cell motility; 6: Cell proliferation; 7: Lipid metabolic process; 8: Metabolic process; 9: Protein folding/metabolism; 10: Signal transduction; 11: Transport.