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. 2014 Jun 11;3(2):616-26.
doi: 10.3390/cells3020616.

Unique Responses are Observed in Transient Receptor Potential Ankyrin 1 and Vanilloid 1 (TRPA1 and TRPV1) Co-Expressing Cells

Laura R Sadofsky  1 , Koti T Sreekrishna  2 , Yakang Lin  3 , Renee Schinaman  4 , Kate Gorka  5 , Yogita Mantri  6 , John Christian Haught  7 , Thomas G Huggins  8 , Robert J Isfort  9 , Charles C Bascom  10 , Alyn H Morice  11
Affiliations

Unique Responses are Observed in Transient Receptor Potential Ankyrin 1 and Vanilloid 1 (TRPA1 and TRPV1) Co-Expressing Cells

Laura R Sadofsky et al. Cells. .

Abstract

Transient receptor potential (TRP) ankyrin 1 (TRPA1) and vanilloid 1 (TRPV1) receptors are implicated in modulation of cough and nociception. In vivo, TRPA1 and TRPV1 are often co-expressed in neurons and TRPA1V1 hetero-tetramer formation is noted in cells co-transfected with the respective expression plasmids. In order to understand the impact of TRP receptor interaction on activity, we created stable cell lines expressing the TRPA1, TRPV1 and co-expressing the TRPA1 and TRPV1 (TRPA1V1) receptors. Among the 600 compounds screened against these receptors, we observed a number of compounds that activated the TRPA1, TRPV1 and TRPA1V1 receptors; compounds that activated TRPA1 and TRPA1V1; compounds that activated TRPV1 and TRPA1V1; compounds in which TRPA1V1 response was modulated by either TRPA1 or TRPV1; and compounds that activated only TRPV1 or TRPA1 or TRPA1V1; and one compound that activated TRPA1 and TRPV1, but not TRPA1V1. These results suggest that co-expression of TRPA1 and TRPV1 receptors imparts unique activation profiles different from that of cells expressing only TRPA1 or TRPV1.

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Figures

Figure 1
Figure 1
TRPV1 cells grown at 37 °C (A) and 33 °C (B).
Figure 2
Figure 2
TRPV1 cells pre-incubated at 25 °C for 30 min, prior to addition of agonist, gives better response.
Figure 3
Figure 3
Examples of compounds that activated all three receptors (TRPA1 = TRPA1V1 > TRPV1).
Figure 4
Figure 4
Examples of compounds that activated all three receptors (TRPA1 < TRPA1V1 = TRPV1).
Figure 5
Figure 5
Examples of compounds that activate TRPA1 = TRPA1V1, but not TRPV1.
Figure 6
Figure 6
Examples of compounds that activate TRPV1 = TRPA1V1, but not TRPA1.
Figure 7
Figure 7
Examples of compounds that activate TRPA1 > TRPA1V1, but not TRPV1.
Figure 8
Figure 8
Examples of compounds that activate TRPV1 > TRPA1V1, but not TRPA1.
Figure 9
Figure 9
Examples of compounds that activate TRPA1 < TRPA1V1, but not TRPV1.
Figure 10
Figure 10
Examples of compounds that activate TRPV1 < TRPA1V1, but not TRPA1.
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