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. 1989 Jan 27;990(1):98-100.
doi: 10.1016/s0304-4165(89)80018-2.

Primary structure of an N-linked sugar chain derived from glucoamylase of Rhizopus niveus

K Takegawa  1 , N Kawasaki, S Iwahara, K Yamamoto, T Tochikura, B Mikami, Y Morita
Affiliations

Primary structure of an N-linked sugar chain derived from glucoamylase of Rhizopus niveus

K Takegawa et al. Biochim Biophys Acta. .

Abstract

The primary structure of the N-linked sugar chain of Rhizopus niveus glucoamylase (major component) was investigated. The carbohydrate moiety was released from the polypeptide backbone by Flavobacterium sp. endo-beta-N-acetylglucosaminidase digestion. Studies using the method of exoglycosidase digestion of the fluorescent pyridylamino derivative, gel-permeation chromatography on Bio-Gel P-4 and 400-MHz 1H-NMR spectroscopy revealed that the most abundant structure is (Man)8-GlcNac-ol.

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