In vitro investigation of antioxidant, anti-Inflammatory, and antiplatelet adhesion properties of genistein-modified poly(ethersulfone)/poly(vinylpyrrolidone) hemodialysis membranes

Abstract

Hemocompatibility of genistein-modified poly(ethersulfone)/poly(vinylpyrrolidone) (PES/PVP) hemodialysis (HD) membranes has been investigated in vitro with emphasis on evaluation of cell viability, antioxidant, anti-inflammatory, and antiplatelet adhesion properties. Genistein modified PES/PVP membranes reveal significant reduction of the reactive oxygen species and also considerable suppression of interleukin-1β and tumor necrosis factor-α levels in whole blood, but to a lesser extent ininterleukin-6. The incorporation of PVP into the HD membrane reduces platelet adhesion by virtue of its hydrophilicity. Of particular importance is that platelet adhesion of the genistein modified membranes declines noticeably at low concentrations of genistein for about 5-10%, beyond which it raises the number of adhered platelets. The initial decline in the platelet adhesion is attributable to genistein's ability to inhibit intercellular and/or vascular cell adhesion, whereas the reversal of this adhesion trend with further increase of genistein loading is ascribed to the inherent hydrophobicity of the genistein modified HD membrane.

Keywords: anti-inflammatory; antioxidant; antiplatelet adhesion; genistein; hemodialysis.