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Review
. 2013 Apr;21(2):143-52.
doi: 10.1016/j.jsps.2012.05.002. Epub 2012 Jun 15.

Review on in vivo and in vitro methods evaluation of antioxidant activity

Md Nur Alam  1 , Nusrat Jahan Bristi  1 , Md Rafiquzzaman  1
Affiliations
Review

Review on in vivo and in vitro methods evaluation of antioxidant activity

Md Nur Alam et al. Saudi Pharm J. 2013 Apr.

Abstract

A good number of abstracts and research articles (in total 74) published, so far, for evaluating antioxidant activity of various samples of research interest were gone through where 407 methods were come across, which were repeated from 29 different methods. These were classified as in vitro and in vivo methods. And those are described and discussed below in this review article. In the later part of this review article, frequency of in vitro as well as in vivo methods is analyzed with a bar diagram. Solvents are important for extracting antioxidants from natural sources. Frequency of solvents used for extraction is also portrayed and the results are discussed in this article. As per this review there are 19 in vitro methods and 10 in vivo methods that are being used for the evaluation of antioxidant activity of the sample of interest. DPPH method was found to be used mostly for the in vitro antioxidant activity evaluation purpose while LPO was found as mostly used in vivo antioxidant assay. Ethanol was with the highest frequency as solvent for extraction purpose.

Keywords: Antioxidant; In vitro methods; In vivo methods.

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Figures

Figure 1
Figure 1
Frequency of commonly used In vitro methods.
Figure 2
Figure 2
Frequency of commonly used In vivo methods.
Figure 3
Figure 3
Commonly used extracting solvents for antioxidant study.
None
None
See this image and copyright information in PMC

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