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. 2014 May;29(3):208-13.
doi: 10.5001/omj.2014.51.

Effect of locally administered ciliary neurotrophic factor on the survival of transected and repaired adult sheep facial nerve

Rashid Al Abri  1 , Arif Ali Kolethekkat  1 , Michael O Kelleher  2 , Lynn M Myles  3 , Michael A Glasby  3
Affiliations

Effect of locally administered ciliary neurotrophic factor on the survival of transected and repaired adult sheep facial nerve

Rashid Al Abri et al. Oman Med J. 2014 May.

Abstract

Objective: to determine whether the administration of Ciliary Neurotrophic Factor (CNTF) at the site of repaired facial nerve enhances regeneration in the adult sheep model.

Methods: Ten adult sheep were divided into 2 groups: control and study group (CNTF group). In the CNTF group, the buccal branch of the facial nerve was transected and then repaired by epineural sutures. CNTF was injected over the left depressor labii maxillaris muscle in the vicinity of the transected and repaired nerve for 28 days under local anesthesia. In the CNTF group, the sheep were again anesthetized after nine months and the site of facial nerve repair was exposed. Detailed electrophysiological, tension experiments and morphometric studies were carried out and then analyzed statistically.

Results: The skin CV min, refractory period, Jitter and tension parameters were marginally raised in the CNTF group than the control but the difference was statistically insignificant between the two groups. Morphometric indices also did not show any significant changes in the CNTF group.

Conclusion: CNTF has no profound effect on neuronal regeneration of adult sheep animal model.

Keywords: CNTF; Neurtrophic factors; Sheep; Facial nerve; Regeneration.

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Figures

Figure 1
Figure 1
Photograph showing the buccal branch of facial nerve in adult sheep.
Figure 2
Figure 2
Box and Whisker plot showing the mean CVmax and CVmin of the control and CNTF groups (SE- standard error, SD- standard deviation)
Figure 3
Figure 3
Box and Whisker plot showing the mean relative and absolute refractory periods of the control and CNTF groups. (RRP- relative refractory period, ARP- absolute refractory period, SE- standard error, SD- standard deviation).
Figure 4
Figure 4
Box and Whisker plot showing area and amplitude of M wave of the control and CNTF groups (SE- standard error, SD- standard deviation).
Figure 5
Figure 5
Box and Whisker plot showing time tension index and mean tetanus of the control and CNTF groups (SE- standard error, SD- standard deviation).
Figure 6
Figure 6
Box and whisker plot comparing the fibre diameter, axon diameter, myelin thickness and g ratio between the control and CNTF groups (SE- standard error, SD- standard deviation).
See this image and copyright information in PMC

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