The Effect of Lactic Acid Bacteria-fermented Soybean Milk Products on Carrageenan-induced Tail Thrombosis in Rats

Abstract

Thrombosis is characterized by congenital and acquired procatarxis. Lactic acid bacteria-fermented soybean milk products (FS-LAB) inhibit hepatic lipid accumulation and prevent atherosclerotic plaque formation. However, the therapeutic efficacy of FS-LAB against thrombosis has yet to be investigated. In this study, FS-LAB were administered subcutaneously into the tails of rats, with the subsequent intravenous administration of κ-carrageenan 12 hr after the initial injection. In general, administration of κ-carrageenan induces thrombosis. The length of the infarcted tail regions was significantly shorter in the rats administered a single-fold or double-fold concentration of the FS-LAB solution compared with the region in control rats. Therefore, FS-LAB exhibited significant antithrombotic effects. Our study is the first to characterize the properties of FS-LAB and, by testing their efficacy on an in vivo rat model of thrombosis, demonstrate the potency of their antithrombotic effect.

Keywords: fermented soybean milk products; lactic acid bacteria; rat tail; thrombosis; κ-carrageenan.

Fig. 1.

Photographs of fibrin clots 8 hr after mixing fibrinogen and thrombin. The concentration of FS-LAB are indicate as 0 ×, 0.1 ×, 0.2 ×, 0.5 ×, 1.0 × and 2.0 ×.

Fig. 2.

Effect of FS-LAB on the mean length of the infarcted tail regions. The horizontal axis shows the time elapsed after the κ-carrageenan injection; κ-carrageenan was injected intravenously 12 hr after subcutaneous injection of FS-LAB solution or PBS. Statistical analysis was performed using ANOVA with a post hoc Tukey test. Significant differences in the mean infarct length between 2× and 1× FS-LAB-administered groups are indicated by *p<0.05. The mean length of the infarcted regions of the FS-LAB-administered groups (2× and 1×) was compared with that of the PBS-administered group at 6, 24 and 48 hr. The PBS column represents the mean value ± SD of 7 measurements. The 1× FS-LAB column represents the mean ± SD of 6 measurements. The 2× FS-LAB column represents the mean value ± SD of 7 measurements.

Fig. 3.

Photographs of infarcted rat tails 24 hr after κ-carrageenan was injected for induction of thrombosis. 2×: rat tail after subcutaneous pre-injection with 2× FS-LAB 12 hr before κ-carrageenan injection. 1×: rat tail after subcutaneous pre-injection with 1× FS-LAB 12 hr before κ-carrageenan injection. PBS: rat tail after subcutaneous pre-injection with PBS 12 hr before κ-carrageenan injection.