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Comparative Study
. 2014 Jun 17:9:101.
doi: 10.1186/1749-8090-9-101.

Immunohistochemistry comparing endoscopic vein harvesting vs. open vein harvesting on saphenous vein endothelium

Mohammad Hassan Nezafati, Pouya Nezafati  1 , Sakineh Amoueian, Armin Attaranzadeh, Hamid Reza Rahimi
Affiliations
Comparative Study

Immunohistochemistry comparing endoscopic vein harvesting vs. open vein harvesting on saphenous vein endothelium

Mohammad Hassan Nezafati et al. J Cardiothorac Surg. .

Abstract

Objective: The present study attempts to compare the immunohistochemistry (IHC) of von Willebrand factor (vWf) , endothelial cadherin, Caveolin and endothelial Nitric Oxide Synthase (eNOS) in VasoView Endoscopic Vein Harvesting (EVH) versus traditional Open Vein Harvesting (OVH) techniques for Coronary Artery Bypass Graft (CABG) Surgery performed in Javad al Aemeh Hospital of Mashhad, Iran in 2013,.

Methods and materials: Forty-seven patients were scheduled for CABG (30 EVH and 17 OVH) among whom patients with relatively same gender and similar age were selected. Three separate two cm vein samples were harvested from each patient's saphenous vein. Each portion was collected from distal, middle and proximal zones of the saphenous vein. The tissues were deparaffinized, and antigen retrieval was done using EZ-retriever followed by an immunohistochemistry evaluation with vWf, e-cadherin, Caveolin and eNOS. In addition, demographic questioner as of Lipid profile, FBS, BMI, and cardiovascular risk factors were collected. Data analyses, including parametric and nonparametric tests were undertaken using the SPSS 16 software. A P value < 0.05 was regarded as statistically significant.

Results: The mean age of the EVH and OVH groups were 63.76 ± 9.51 and 63.63 ± 8.31 years respectively with no significant difference between them (p = 0.989). In addition, there was no great difference between the EVH and OVH groups in lipid profile, DM, HTN, smoking history, CVA, and valvular dysfunction (P > 0.05). Qualitative report of vWf, e-cadherin, Caveolin and eNOS reveals no significant difference between the EVH and OVH (P > 0.05).

Conclusion: This study indicates that VasoView EVH technique causes no endothelial damage in comparison with OVH. This study could be a molecular confirmation for the innocuous of EVH technique.

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Figures

Figure 1
Figure 1
EVH and OVH procedure (EVH at right and OVH at left).
Figure 2
Figure 2
eNOS, vWF, e-cadherin, and ccaveolin in EVH and OVH immunohistochemistry slides. Qualitative assessing of slides were done by a same expert pathologist, as micrographs showed same staining intensity in intercellular and intracellular space.
See this image and copyright information in PMC

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