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. 2014 Jul;8(1):159-164.
doi: 10.3892/etm.2014.1674. Epub 2014 Apr 8.

Monoamine neurotransmitters and fibroblast growth factor-2 in the brains of rats with post-stroke depression

Xiao-Wei Ji  1 , Chun-Ling Wu  2 , Xing-Chen Wang  1 , Jie Liu  1 , Jian-Zhong Bi  3 , Dian-Yun Wang  4
Affiliations

Monoamine neurotransmitters and fibroblast growth factor-2 in the brains of rats with post-stroke depression

Xiao-Wei Ji et al. Exp Ther Med. 2014 Jul.

Abstract

The aim of the present study was to investigate the changes in the levels of serotonin (5-HT), dopamine (DA), norepinephrine (NE) and fibroblast growth factor-2 (FGF-2) in the brains of rats with post-stroke depression (PSD). A rat model of stroke was established by middle cerebral artery occlusion and the rats were randomly divided into two groups: Control and modification groups. The rats in the modification group had PSD, while the rats in the control group had experienced a stroke only. The PSD model was established by applying chronic mild stress to the individually housed rats. High-performance liquid chromatography was used to detect the levels of 5-HT, DA and NE, while western blotting was used to detect the FGF-2 protein expression levels in the frontal lobe and hippocampus. Quantitative polymerase chain reaction was also used to determine the mRNA expression levels of FGF-2 in the frontal lobes of the two groups. The levels of 5-HT, DA and NE in the frontal lobe and hippocampus of the rats in the PSD group were significantly lower than the levels observed in the rats in the stroke group (P<0.01). In addition, protein expression levels of FGF-2 in the frontal lobe of the rats in the PSD group were significantly lower when compared with the control group rats (P<0.01), however, the protein expression levels of FGF-2 in the hippocampus did not exhibit a statistically significant difference (P>0.05). The mRNA expression levels of FGF-2 in the frontal lobe of the rats in the modification group were significantly lower than the levels in the control group rats (P<0.01). Therefore, reduced levels of monoamine neurotransmitters and FGF-2 expression in the brains of rats with PSD are associated with the incidence of PSD.

Keywords: dopamine; fibroblast growth factor-2; norepinepherine; post-stroke depression rat; serotonin.

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Figures

Figure 1
Figure 1
Protein expression levels of FGF-2 in the frontal lobe tissue. Lanes 1–6 represent individual rats in the same group. The bar graph shows the average number of PSD and STROKE in each condition. Results are presented as the mean ± standard deviation from six individual rats chosen at random. #P<0.01, PSD vs. stroke. FGF-2, fibroblast growth factor-2; PSD, post-stroke depression; STROKE, control group.
Figure 2
Figure 2
Protein expression levels of FGF-2 in the hippocampus. Lanes 1–6 represents a single individual in the same group. The bar graph shows the average number of PSD and STROKE in each condition. Results are presented as the mean ± standard deviation from six individual rats chosen at random. FGF-2, fibroblast growth factor-2; PSD, post-stroke depression; STROKE, control group.
Figure 3
Figure 3
mRNA expression levels of FGF-2 in the frontal lobe tissue. #P<0.01, PSD vs. stroke. FGF-2, fibroblast growth factor-2; PSD, post-stroke depression.
See this image and copyright information in PMC

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