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. 2014 Jul;8(1):175-180.
doi: 10.3892/etm.2014.1685. Epub 2014 Apr 14.

Detection of serum VEGF and MMP-9 levels by Luminex multiplexed assays in patients with breast infiltrative ductal carcinoma

Junying Zhang  1 , Li Yin  2 , Jianzhong Wu  3 , Ye Zhang  3 , Tao Xu  4 , Rong Ma  3 , Haixia Cao  3 , Jinhai Tang  4
Affiliations

Detection of serum VEGF and MMP-9 levels by Luminex multiplexed assays in patients with breast infiltrative ductal carcinoma

Junying Zhang et al. Exp Ther Med. 2014 Jul.

Abstract

The aim of the present study was to assess the effect of the combined detection of serum vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9) by Luminex multiplexed assays for the diagnosis, treatment and prognosis of breast cancer. Preoperative levels of serum VEGF and MMP-9 were detected via a lipid chip-based method in 301 breast cancer cases, 83 breast fibroadenoma cases and 40 healthy adults. Postoperative levels of VEGF and MMP-9 were also detected in 118 breast cancer cases. The levels of serum VEGF and MMP-9 in patients with breast infiltrative ductal carcinoma (IDC) were higher than those in the breast fibroadenoma and healthy control groups (P<0.05); there was no statistically significant difference between the breast fibroadenoma and healthy groups (P>0.05). The levels of VEGF and MMP-9 were shown to correlate with the clinical stage, tumor size and the lymph node metastasis status. However, the levels were not associated with age or gender (P>0.05). In addition, the serum level of MMP-9 exhibited a significantly correlation with the VEGF level (r=0.601, P<0.001). Subgroup analysis revealed that in patients with IDC, serum levels of VEGF and MMP-9 prior to surgery were significantly higher than those following surgery (P<0.05). Therefore, the serum levels of VEGF and MMP-9 can be used as markers for the diagnosis of breast IDC and may also be valuable for the prediction of lymph nodes metastasis.

Keywords: breast infiltrative ductal carcinoma; liquid chip-based method; matrix metalloproteinase-9; vascular endothelial growth factor.

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Figures

Figure 1
Figure 1
Comparisons in the levels of serum (A) VEGF and (B) MMP-9 among IDC, fibroadenoma and healthy individuals. VEGF, vascular endothelial growth factor; MMP-9, matrix metalloproteinase-9; IDC, infiltrative ductal carcinoma.
Figure 2
Figure 2
Comparison in the levels of serum (A) VEGF and (B) MMP-9 between preoperative and postoperative patients with IDC. VEGF, vascular endothelial growth factor; MMP-9, matrix metalloproteinase-9; IDC, infiltrative ductal carcinoma.
Figure 3
Figure 3
Correlation between serum VEGF and MMP-9 levels in IDC patients. VEGF, vascular endothelial growth factor; MMP-9, matrix metalloproteinase-9; IDC, infiltrative ductal carcinoma.
Figure 4
Figure 4
Area under the ROC curves for the levels of serum (A) VEGF and (B) MMP-9 in IDC patients and healthy individuals. ROC, receiver operating characteristic; VEGF, vascular endothelial growth factor; MMP-9, matrix metalloproteinase-9; IDC, infiltrative ductal carcinoma.
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