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Case Reports
. 2014 Sep;52(9):3414-7.
doi: 10.1128/JCM.01271-14. Epub 2014 Jun 20.

Cluster of Escherichia coli isolates producing a plasmid-mediated OXA-48 β-lactamase in a Spanish hospital in 2012

Javier Fernández  1 , Ignacio Montero  2 , Ana Fleites  3 , M Rosario Rodicio  4
Affiliations
Case Reports

Cluster of Escherichia coli isolates producing a plasmid-mediated OXA-48 β-lactamase in a Spanish hospital in 2012

Javier Fernández et al. J Clin Microbiol. 2014 Sep.

Abstract

Three unrelated sequence type 131 (ST131), ST58, and ST83 Escherichia coli isolates with low-level resistance to imipenem and resistance to ertapenem were recovered in a Spanish hospital from July to October 2012. They were positive for blaOXA-48 carried by an IncL/M conjugative plasmid, which may have been acquired from Klebsiella pneumoniae.

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Figures

FIG 1
FIG 1
Plasmid profiles of Klebsiella pneumoniae (Kp) and Escherichia coli (Ec) isolates producing OXA-48 carbapenemase (a) and hybridization with blaOXA-48 (b) and blaCTX-M-15 (c) probes. Lanes λ, phage λ DNA digested with PstI, used as reference for the hybridization experiments (the sizes of some of the linear fragments are shown in parentheses); lanes i and ii, plasmids obtained from E. coli V517 (NCTC 50192) and plasmid RP4 used as molecular size standards for undigested DNA; lanes 1, Kp-HUCA 4; lanes 2, Ec-HUCA 1; lanes 3, Ec-HUCA 2; lanes 4, Ec-HUCA 3.
FIG 2
FIG 2
XbaI pulsed-field electrophoresis profiles of Klebsiella pneumoniae (Kp) and Escherichia coli (Ec) isolates producing OXA-48 carbapenemase. Lane C, XbaI-digested DNA of Salmonella enterica serovar Braenderup H9812 used as size standard; lane 1, Kp-HUCA 4; lane 2, Ec-HUCA 1; lane 3, Ec-HUCA 2; lane 4, Ec-HUCA 3.
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References

    1. Patel G, Bonomo RA. 2013. “Stormy waters ahead”: global emergence of carbapenemases. Front. Microbiol. 4:48. 10.3389/fmicb.2013.00048. - DOI - PMC - PubMed
    1. Nordmann P, Dortet L, Poirel L. 2012. Carbapenem resistance in Enterobacteriaceae: here is the storm! Trends Mol. Med. 18:263–272. 10.1016/j.molmed.2012.03.003. - DOI - PubMed
    1. Poirel L, Potron A, Nordmann P. 2012. OXA-48-like carbapenemases: the phantom menace. J. Antimicrob. Chemother. 67:1597–1606. 10.1093/jac/dks121. - DOI - PubMed
    1. Canton R, Akova M, Carmeli Y, Giske CG, Glupczynski Y, Gniadkowski M, Livermore DM, Miriagou V, Naas T, Rossolini GM, Samuelsen O, Seifert H, Woodford N, Nordmann P, European Network on Carbapenemases 2012. Rapid evolution and spread of carbapenemases among Enterobacteriaceae in Europe. Clin. Microbiol. Infect. 18:413–431. 10.1111/j.1469-0691.2012.03821.x. - DOI - PubMed
    1. Lascols C, Peirano G, Hackel M, Laupland KB, Pitout JD. 2013. Surveillance and molecular epidemiology of Klebsiella pneumoniae isolates that produce carbapenemases: first report of OXA-48-like enzymes in North America. Antimicrob. Agents Chemother. 57:130–136. 10.1128/AAC.01686-12. - DOI - PMC - PubMed

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