Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014 Apr 15;7(4):919-26.
eCollection 2014.

A novel fluid resuscitation protocol: provide more protection on acute kidney injury during septic shock in rats

Wei Liu  1 , Xue-Song Dong  1 , Yu-Qiang Sun  1 , Zhi Liu  1
Affiliations

A novel fluid resuscitation protocol: provide more protection on acute kidney injury during septic shock in rats

Wei Liu et al. Int J Clin Exp Med. .

Abstract

Objective: To investigate the therapeutic effects of a novel fluid resuscitation protocol (early fluid resuscitation plus 2% hydrogen inhalation) on acute kidney injury during septic shock induced by lipopolysaccharide in rats.

Methods: Sixty male Wistar rats were randomly divided into four groups (n = 15 per group): control group (C), septic shock group (S), septic shock with early fluid resuscitation group (R), and septic shock with early fluid resuscitation plus 2% hydrogen inhalation group (R+R+H2). The rats were ventilated, and a 2% hydrogen mixture was used in Group R+H2. Lipopolysaccharide (10 mg/kg) was administered to establish the septic shock model in rats and fluid resuscitation was performed in Groups R and R+R+H2.

Results: Fluid resuscitation with 2% hydrogen inhalation decreased serum creatinine, blood urea nitrogen, and neutrophil gelatinase-associated lipocalin. It also reduced oxidative stress injury and decreased renal tumor necrosis factor-α and interleukin-6 levels compared with fluid resuscitation alone.

Conclusion: Early fluid resuscitation plus 2% hydrogen inhalation provided more protection against AKI during septic shock.

Keywords: Septic shock; acute kidney injury; fluid resuscitation; hydrogen inhalation; inflammation; oxidative stress.

PubMed Disclaimer

Figures

Figure 1
Figure 1
A. Serum Cr in different groups; B. Serum BUN in different groups; C. NGAL levels in different groups. Data are expressed as mean ± SD, n = 15. ﹟p < 0.05 versus Group C; Δp < 0.05 versus Group S; †p < 0.05 versus Group R.
Figure 2
Figure 2
A. Photomicrographs of kidney sections of different groups. Routine hematoxylin and eosin stained (200×). B. Transmission electron microscopies of glomerular filtration membrane of different groups, (TEM 5000×, Bar scale = 1 μm).
Figure 3
Figure 3
A. MPO expression of different groups; B. MDA expression of different groups; C. SOD expression of different groups. Data are expressed as mean ± SD, n = 15. ﹟p < 0.05 versus Group C; Δp < 0.05 versus Group S; †p < 0.05 versus Group R.
Figure 4
Figure 4
A. TNF-α levels of different groups; B. IL-6 levels of different groups; C. IL-10 levels of different groups; D. Ratio of IL-6/IL-10 of different groups. Data are expressed as mean ± SD, n = 15. ﹟p < 0.05 versus Group C; Δp < 0.05 versus Group S; †p < 0.05 versus Group R.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Angus DC, Linde-Zwirble WT, Lidicker J, Clermont G, Carcillo J, Pinsky MR. Epidemiology of severe sepsis in the United States: analysis of incidence outcome and associated costs of care. Crit Care Med. 2001;29:1303–1310. - PubMed
    1. Martin GS, Mannino DM, Eaton S. The epidemiology of sepsis in the United States from 1979 through 2000. N Engl J Med. 2003;348:1546–1554. - PubMed
    1. Rivers E, Nguyen B, Havstad S. Early goal-directed therapy in the treatment of severe sepsis and septic shock. N Engl J Med. 2001;345:1368–1377. - PubMed
    1. Trzeciak S, McCoy JV, Phillip Dellinger R. Early increases in microcirculatory perfusion during protocol directed resuscitation are associated with reduced multi-organ failure at 24 h in patients with sepsis. Intensive Care Med. 2008;34:2210–2217. - PMC - PubMed
    1. Jones AE, Focht A, Horton JM, Kline JA. Prospective external validation of the clinical effectiveness of an emergency department-based early goal-directed therapy protocol for severe sepsis and septic shock. Chest. 2007;132:425–432. - PMC - PubMed

LinkOut - more resources