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. 2014 Apr 15;7(4):919-26.
eCollection 2014.

A novel fluid resuscitation protocol: provide more protection on acute kidney injury during septic shock in rats

Wei Liu  1 , Xue-Song Dong  1 , Yu-Qiang Sun  1 , Zhi Liu  1
Affiliations

A novel fluid resuscitation protocol: provide more protection on acute kidney injury during septic shock in rats

Wei Liu et al. Int J Clin Exp Med. .

Abstract

Objective: To investigate the therapeutic effects of a novel fluid resuscitation protocol (early fluid resuscitation plus 2% hydrogen inhalation) on acute kidney injury during septic shock induced by lipopolysaccharide in rats.

Methods: Sixty male Wistar rats were randomly divided into four groups (n = 15 per group): control group (C), septic shock group (S), septic shock with early fluid resuscitation group (R), and septic shock with early fluid resuscitation plus 2% hydrogen inhalation group (R+R+H2). The rats were ventilated, and a 2% hydrogen mixture was used in Group R+H2. Lipopolysaccharide (10 mg/kg) was administered to establish the septic shock model in rats and fluid resuscitation was performed in Groups R and R+R+H2.

Results: Fluid resuscitation with 2% hydrogen inhalation decreased serum creatinine, blood urea nitrogen, and neutrophil gelatinase-associated lipocalin. It also reduced oxidative stress injury and decreased renal tumor necrosis factor-α and interleukin-6 levels compared with fluid resuscitation alone.

Conclusion: Early fluid resuscitation plus 2% hydrogen inhalation provided more protection against AKI during septic shock.

Keywords: Septic shock; acute kidney injury; fluid resuscitation; hydrogen inhalation; inflammation; oxidative stress.

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Figures

Figure 1
Figure 1
A. Serum Cr in different groups; B. Serum BUN in different groups; C. NGAL levels in different groups. Data are expressed as mean ± SD, n = 15. ﹟p < 0.05 versus Group C; Δp < 0.05 versus Group S; †p < 0.05 versus Group R.
Figure 2
Figure 2
A. Photomicrographs of kidney sections of different groups. Routine hematoxylin and eosin stained (200×). B. Transmission electron microscopies of glomerular filtration membrane of different groups, (TEM 5000×, Bar scale = 1 μm).
Figure 3
Figure 3
A. MPO expression of different groups; B. MDA expression of different groups; C. SOD expression of different groups. Data are expressed as mean ± SD, n = 15. ﹟p < 0.05 versus Group C; Δp < 0.05 versus Group S; †p < 0.05 versus Group R.
Figure 4
Figure 4
A. TNF-α levels of different groups; B. IL-6 levels of different groups; C. IL-10 levels of different groups; D. Ratio of IL-6/IL-10 of different groups. Data are expressed as mean ± SD, n = 15. ﹟p < 0.05 versus Group C; Δp < 0.05 versus Group S; †p < 0.05 versus Group R.
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